PMID- 10464163
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20190513
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 11
IP  - 9
DP  - 1999 Sep
TI  - Prf, a novel Ets family protein that binds to the PU.1 binding motif, is
      specifically expressed in restricted stages of B cell development.
PG  - 1423-9
AB  - During the development of lymphocytes, expression of the Ig genes is strictly
      regulated in a tissue-specific manner and in a time-ordered fashion. We have
      previously shown that the PU.1 binding motif in the Igkappa 3' enhancer
      (kappaE3') and a novel Ets family protein other than PU.1 may be possibly
      involved in the control of V(kappa)-J(kappa) joining. In the attempt to isolate
      the novel Ets family protein, we have screened cDNA libraries with the yeast
      one-hybrid method and identified a new PU.1-related factor, Prf. This novel Ets
      family protein is shown to interact with the PU.1 binding sequences in various
      promoters and enhancers, including kappaE3'. It was found that expression of the 
      prf gene is predominant in the B-lineage cells, with the exception of immature B 
      cells. Since Prf does not exhibit functions of transcriptional activity, this
      novel protein may act as an antagonist against other Ets family proteins, e.g.
      PU.1 and Spi-B. Possible roles of Prf with respect to the B cell differentiation 
      are discussed.
FAU - Hashimoto, S
AU  - Hashimoto S
AD  - Department of Biophysics and Biochemistry, Graduate School of Science, University
      of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Nishizumi, H
AU  - Nishizumi H
FAU - Hayashi, R
AU  - Hayashi R
FAU - Tsuboi, A
AU  - Tsuboi A
FAU - Nagawa, F
AU  - Nagawa F
FAU - Takemori, T
AU  - Takemori T
FAU - Sakano, H
AU  - Sakano H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (RNA, Messenger)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (proto-oncogene protein Spi-1)
RN  - 148350-00-9 (SPIB protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - B-Lymphocytes/*chemistry/cytology
MH  - Base Sequence
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation/drug effects
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Enhancer Elements, Genetic/genetics
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins/*chemistry
MH  - Proto-Oncogene Proteins c-ets
MH  - RNA, Messenger/analysis
MH  - Spleen/cytology
MH  - Thymus Gland/cytology
MH  - Trans-Activators/*chemistry
MH  - Transcription Factors/*chemistry/genetics
EDAT- 1999/08/28 00:00
MHDA- 1999/08/28 00:01
CRDT- 1999/08/28 00:00
PHST- 1999/08/28 00:00 [pubmed]
PHST- 1999/08/28 00:01 [medline]
PHST- 1999/08/28 00:00 [entrez]
AID - 10.1093/intimm/11.9.1423 [doi]
PST - ppublish
SO  - Int Immunol. 1999 Sep;11(9):1423-9. doi: 10.1093/intimm/11.9.1423.