PMID- 10464099
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5432
DP  - 1999 Aug 27
TI  - GTP binding by class II transactivator: role in nuclear import.
PG  - 1402-5
AB  - Class II transactivator (CIITA) is a global transcriptional coactivator of human 
      leukocyte antigen-D (HLA-D) genes. CIITA contains motifs similar to guanosine
      triphosphate (GTP)-binding proteins. This report shows that CIITA binds GTP, and 
      mutations in these motifs decrease its GTP-binding and transactivation activity. 
      Substitution of these motifs with analogous sequences from Ras restores CIITA
      function. CIITA exhibits little GTPase activity, yet mutations in CIITA that
      confer GTPase activity reduce transcriptional activity. GTP binding by CIITA
      correlates with nuclear import. Thus, unlike other GTP-binding proteins, CIITA is
      involved in transcriptional activation that uses GTP binding to facilitate its
      own nuclear import.
FAU - Harton, J A
AU  - Harton JA
AD  - Lineberger Comprehensive Cancer Center, University of North Carolina-Chapel Hill,
      Chapel Hill, NC 27599, USA.
FAU - Cressman, D E
AU  - Cressman DE
FAU - Chin, K C
AU  - Chin KC
FAU - Der, C J
AU  - Der CJ
FAU - Ting, J P
AU  - Ting JP
LA  - eng
GR  - AI29564/AI/NIAID NIH HHS/United States
GR  - AI41751/AI/NIAID NIH HHS/United States
GR  - AI45580/AI/NIAID NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (HLA-DR Antigens)
RN  - 0 (MHC class II transactivator protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
CIN - Science. 1999 Aug 27;285(5432):1367. PMID: 10490413
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Binding Sites
MH  - COS Cells
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - GTP-Binding Proteins/chemistry/genetics/*metabolism
MH  - *Genes, MHC Class II
MH  - Guanosine Triphosphate/*metabolism
MH  - HLA-DR Antigens/genetics
MH  - Humans
MH  - Mutation
MH  - *Nuclear Proteins
MH  - Promoter Regions, Genetic
MH  - Temperature
MH  - Trans-Activators/chemistry/genetics/*metabolism
MH  - Transcription Factors/metabolism
MH  - *Transcriptional Activation
EDAT- 1999/08/28 00:00
MHDA- 1999/08/28 00:01
CRDT- 1999/08/28 00:00
PHST- 1999/08/28 00:00 [pubmed]
PHST- 1999/08/28 00:01 [medline]
PHST- 1999/08/28 00:00 [entrez]
AID - 7772 [pii]
AID - 10.1126/science.285.5432.1402 [doi]
PST - ppublish
SO  - Science. 1999 Aug 27;285(5432):1402-5. doi: 10.1126/science.285.5432.1402.