PMID- 10464096
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5432
DP  - 1999 Aug 27
TI  - Dual function of the selenoprotein PHGPx during sperm maturation.
PG  - 1393-6
AB  - The selenoprotein phospholipid hydroperoxide glutathione peroxidase (PHGPx)
      changes its physical characteristics and biological functions during sperm
      maturation. PHGPx exists as a soluble peroxidase in spermatids but persists in
      mature spermatozoa as an enzymatically inactive, oxidatively cross-linked,
      insoluble protein. In the midpiece of mature spermatozoa, PHGPx protein
      represents at least 50 percent of the capsule material that embeds the helix of
      mitochondria. The role of PHGPx as a structural protein may explain the
      mechanical instability of the mitochondrial midpiece that is observed in selenium
      deficiency.
FAU - Ursini, F
AU  - Ursini F
AD  - Dipartmento di Chimica Biologica, Universita di Padova, Viale G. Colombo 3,
      I-35121 Padova, Italy.
FAU - Heim, S
AU  - Heim S
FAU - Kiess, M
AU  - Kiess M
FAU - Maiorino, M
AU  - Maiorino M
FAU - Roveri, A
AU  - Roveri A
FAU - Wissing, J
AU  - Wissing J
FAU - Flohe, L
AU  - Flohe L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Proteins)
RN  - 0 (Selenoproteins)
RN  - EC 1.11.1.12 (phospholipid-hydroperoxide glutathione peroxidase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - H6241UJ22B (Selenium)
SB  - IM
CIN - Science. 1999 Aug 27;285(5432):1339. PMID: 10490404
MH  - Animals
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glutathione Peroxidase/chemistry/isolation & purification/*physiology
MH  - Infertility, Male/metabolism
MH  - Male
MH  - Mitochondria/chemistry/enzymology
MH  - Oxidation-Reduction
MH  - Proteins/chemistry/isolation & purification/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Selenium/deficiency/*physiology
MH  - Selenoproteins
MH  - Solubility
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spermatids/chemistry/enzymology
MH  - *Spermatogenesis
MH  - Spermatozoa/chemistry/enzymology/*physiology
EDAT- 1999/08/28 00:00
MHDA- 1999/08/28 00:01
CRDT- 1999/08/28 00:00
PHST- 1999/08/28 00:00 [pubmed]
PHST- 1999/08/28 00:01 [medline]
PHST- 1999/08/28 00:00 [entrez]
AID - 7779 [pii]
AID - 10.1126/science.285.5432.1393 [doi]
PST - ppublish
SO  - Science. 1999 Aug 27;285(5432):1393-6. doi: 10.1126/science.285.5432.1393.