PMID- 10462485
OWN - NLM
STAT- MEDLINE
DCOM- 19991001
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 2
DP  - 1999 Aug 27
TI  - Identification and characterization of human Fas associated factor 1, hFAF1.
PG  - 388-94
AB  - We have identified and characterized a cDNA encoding human Fas associated factor 
      1 (hFAF1) cDNA and a shorter form of hFAF1 cDNA [hFAF1(s)] with a 456 bp internal
      in-frame deletion from a human HeLa cDNA library. The nucleotide sequences of
      hFAF1 and hFAF1(s) were identical except for the deletion. GST-hFAF1 fusion
      protein bound to the in vitro translation product of Fas. The N-terminal region
      (amino acid 1 approximately 201) including the upstream ubiquitin homology domain
      of hFAF1 could bind with the death domain of Fas unlike that of qFAF1 whose
      binding region with Fas could not be determined. However hFAF1 did not bind to
      the death domain of Fas mutant, lpr(cg). hFAF1 was expressed abundantly in
      testis, skeletal muscle, and heart as 2.8 kb mRNA. Polyclonal antibody against
      hFAF1 detected 74 kD protein, a deduced protein size from the ORF and 40 kD
      protein in some cell lines.
CI  - Copyright 1999 Academic Press.
FAU - Ryu, S W
AU  - Ryu SW
AD  - Division of Life Science, PaiChai University, 439-6 Doma-dong, Seo-gu, Taejon,
      302-735, South Korea.
FAU - Chae, S K
AU  - Chae SK
FAU - Lee, K J
AU  - Lee KJ
FAU - Kim, E
AU  - Kim E
LA  - eng
SI  - GENBANK/AF094700
SI  - GENBANK/AF106798
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (FAF1 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Apoptosis
MH  - Base Sequence
MH  - Binding Sites
MH  - Carrier Proteins/*genetics/*metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Recombinant Proteins/metabolism
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - fas Receptor/genetics/*metabolism
EDAT- 1999/08/27 00:00
MHDA- 1999/08/27 00:01
CRDT- 1999/08/27 00:00
PHST- 1999/08/27 00:00 [pubmed]
PHST- 1999/08/27 00:01 [medline]
PHST- 1999/08/27 00:00 [entrez]
AID - 10.1006/bbrc.1999.1217 [doi]
AID - S0006-291X(99)91217-2 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 27;262(2):388-94. doi:
      10.1006/bbrc.1999.1217.