PMID- 10461921
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 3
DP  - 1999 Sep
TI  - Processing of rat preprocortistatin in mouse AtT-20 cells.
PG  - 1273-7
AB  - Preprocortistatin (PPCST) has been recently identified as a novel somatostatin
      (SST)-related gene expressed only in brain. PPCST shares 11 of 14 residues with
      SST-14 at its C-terminal segment, where it features Lys-Lys and Lys-Arg basic
      sites for cleavage to putative cortistatin (CST)-14 and CST-29 peptides,
      respectively. Although synthetic replicates of the two putative CST peptides
      interact with SST receptors, they also display novel effects suggesting
      independent biological functions. Nothing is currently known about the naturally 
      occurring mature cleavage products of PPCST posttranslational processing. Here we
      have cloned rat PPCST cDNA, stably expressed it in AtT-20 pituitary cells, and
      characterized the cellular and releasable products of PPCST processing by HPLC
      and radioimmunoassay using a SST-14 antibody that recognizes synthetic CST-14 and
      CST-29. Transfected cells released 120 +/- 21 pg of total CST-LI per plate
      basally, with an increase to 204 +/- 33 pg per plate with forskolin stimulation
      (p < 0.05). HPLC chromatograms of cell extracts revealed three peaks
      corresponding to CST-14, CST-29, and unprocessed PPCST (ratio, 41:55:4.5). CST
      was released preferentially as CST-14 (63-70%) compared with CST-29 (30-37%)
      under basal and forskolin-stimulated conditions. These studies demonstrate
      efficient processing of PPCST to both CST-14 and CST-29 through putative cleavage
      at both C-terminal dibasic sites of PPCST. Although the two peptides are
      synthesized approximately equally, CST-14 is released preferentially via the
      regulated secretory pathway.
FAU - Puebla, L
AU  - Puebla L
AD  - Department of Medicine, Royal Victoria Hospital, Montreal, Quebec, Canada.
FAU - Mouchantaf, R
AU  - Mouchantaf R
FAU - Sasi, R
AU  - Sasi R
FAU - Khare, S
AU  - Khare S
FAU - Bennett, H P
AU  - Bennett HP
FAU - James, S
AU  - James S
FAU - Patel, Y C
AU  - Patel YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (DNA, Complementary)
RN  - 0 (Protein Precursors)
RN  - 0 (preprocortistatin)
RN  - 1F7A44V6OU (Colforsin)
RN  - 51110-01-1 (Somatostatin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromatography, High Pressure Liquid
MH  - Colforsin/pharmacology
MH  - DNA, Complementary/biosynthesis/genetics
MH  - Mice
MH  - Pituitary Gland/cytology/metabolism
MH  - Protein Precursors/*biosynthesis/genetics
MH  - Radioimmunoassay
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Somatostatin/biosynthesis
MH  - Transfection
EDAT- 1999/08/26 00:00
MHDA- 1999/08/26 00:01
CRDT- 1999/08/26 00:00
PHST- 1999/08/26 00:00 [pubmed]
PHST- 1999/08/26 00:01 [medline]
PHST- 1999/08/26 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0731273.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Sep;73(3):1273-7. doi: 10.1046/j.1471-4159.1999.0731273.x.