PMID- 10460833
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20200403
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 9
DP  - 1999 Sep
TI  - Characterization of human vascular endothelial cadherin glycans.
PG  - 915-25
AB  - The glycosylation pattern of human vascular endothelial cadherin (VE-cadherin),
      purified from cultured human umbilical cord vein endothelial cells, was analyzed.
      VE-cadherin was metabolically radiolabeled with d-[6-(3)H]glucosamine, isolated
      by immunoprecipitation, purified by SDS-PAGE and in-gel digested with
      endoproteinase Asp N. Oligosaccharides were sequentially released from resulting 
      glycopeptides and analyzed by chromatographic profiling. The results revealed
      that VE-cadherin carries predominantly sialylated diantennary and hybrid-type
      glycans in addition to some triantennary and high mannose-type species. Highly
      branched, tetraantennary oligosaccharides were found in trace amounts only.
      Immunohistochemical labeling of VE-cadherin and sialic acids displayed a
      codistribution along the intercellular junctions in endothelial cells of human
      umbilical arteries, veins, and cultured endothelial monolayers. Ca(2+)-depletion,
      performed on cultured endothelial cells, resulted in a reversible complete
      disappearance of VE-cadherin and of almost all sialic acid staining from the
      junctions. Sialidase treatment of whole cells caused a change of VE-cadherin
      immunofluorescence from a continuous and netlike superstructural organization to 
      a scattered inconsistent one. Hence, cell surface sialic acids might play a role 
      in VE-cadherin organization.
FAU - Geyer, H
AU  - Geyer H
AD  - Institute of Biochemistry, Justus-Liebig-Universitat Giessen, Friedrichstrasse
      24, D-35392 Giessen, Germany.
FAU - Geyer, R
AU  - Geyer R
FAU - Odenthal-Schnittler, M
AU  - Odenthal-Schnittler M
FAU - Schnittler, H J
AU  - Schnittler HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Actins)
RN  - 0 (Antigens, CD)
RN  - 0 (Cadherins)
RN  - 0 (Glycoproteins)
RN  - 0 (Oligosaccharides)
RN  - 0 (cadherin 5)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Actins/isolation & purification
MH  - Antigens, CD
MH  - Cadherins/*chemistry
MH  - Carbohydrate Sequence
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*chemistry
MH  - Glycoproteins/*chemistry
MH  - Humans
MH  - Intercellular Junctions/chemistry
MH  - Molecular Sequence Data
MH  - N-Acetylneuraminic Acid/isolation & purification
MH  - Oligosaccharides/*chemistry
MH  - Umbilical Veins/cytology
PMC - PMC7108604
EDAT- 1999/08/26 00:00
MHDA- 1999/08/26 00:01
CRDT- 1999/08/26 00:00
PHST- 1999/08/26 00:00 [pubmed]
PHST- 1999/08/26 00:01 [medline]
PHST- 1999/08/26 00:00 [entrez]
AID - cwc092 [pii]
AID - 10.1093/glycob/9.9.915 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Sep;9(9):915-25. doi: 10.1093/glycob/9.9.915.