PMID- 10460751
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20101118
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 21
IP  - 3
DP  - 1999 Sep
TI  - Selective expression of RT6 superfamily in human bronchial epithelial cells.
PG  - 337-46
AB  - RT6 proteins are glycosylphosphatidylinositol (GPI)-linked alloantigens that are 
      localized to cytotoxic T lymphocytes and that have nicotinamide adenine
      dinucleotide glycohydrolase and adenosine diphosphate (ADP)-ribosyltransferase
      activities. In view of the importance of GPI-linked surface proteins in mediating
      interactions of cells with their milieu, and the varied functions of airway cells
      in inflammation, we undertook the present study to determine whether human
      homologues of the RT6 superfamily of ADP-ribosyltransferases (ART) are expressed 
      in pulmonary epithelial cells. We hypothesized that these surface proteins or
      related family members may be present in cells that interact with inflammatory
      cells, and that they may thereby be involved in intercellular signaling. Using in
      situ analysis and Northern blot analysis, we identified ART1 messenger RNA (mRNA)
      in airway epithelial cells. As expected for GPI-anchored proteins, the
      localization of ART1 at the apical surface of ciliated epithelial cells was
      demonstrated by staining with polyclonal anti-ART1 antibody, and was confirmed by
      loss of this immunoreactivity after treatment with phosphatidylinositol-specific 
      phospholipase C (PI-PLC), which selectively cleaves GPI anchors and releases
      proteins from the plasma membrane. Using in situ hybridization with specific ART3
      and ART4 oligonucleotides, we also identified two additional members of the RT6
      superfamily in epithelial cells. In accord with these findings, we identified
      ART3 and ART4 mRNAs through reverse transcription- polymerase chain reaction of
      polyadenine-positive RNA from human trachea. Interestingly, these proteins
      appeared to be preferentially localized to the airway epithelium. The localized
      expression of these members of the RT6 superfamily in human pulmonary epithelial 
      cells may reflect a role for them in cell-cell signaling during immune responses 
      within the airway.
FAU - Balducci, E
AU  - Balducci E
AD  - Pulmonary-Critical Care Medicine Branch and Pathology Section, National Heart,
      Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland,
      USA.
FAU - Horiba, K
AU  - Horiba K
FAU - Usuki, J
AU  - Usuki J
FAU - Park, M
AU  - Park M
FAU - Ferrans, V J
AU  - Ferrans VJ
FAU - Moss, J
AU  - Moss J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SWI4 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.4.2.- (ADP Ribose Transferases)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 2.4.2.31 (ART1 protein, human)
RN  - EC 2.4.2.31 (ART3 protein, human)
RN  - EC 2.4.2.31 (ART4 protein, human)
RN  - EC 2.4.2.31 (Art2b protein, rat)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.11 (Phosphoinositide Phospholipase C)
RN  - EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
SB  - IM
MH  - ADP Ribose Transferases/analysis/*metabolism
MH  - Antigens, Differentiation, T-Lymphocyte
MH  - Bronchi/anatomy & histology/*metabolism
MH  - DNA-Binding Proteins
MH  - Epithelial Cells/metabolism
MH  - Fungal Proteins/analysis/metabolism
MH  - GPI-Linked Proteins
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Lung/anatomy & histology
MH  - Membrane Glycoproteins/analysis/*metabolism
MH  - Membrane Proteins/analysis/metabolism
MH  - Multigene Family
MH  - Phosphatidylinositol Diacylglycerol-Lyase
MH  - Phosphoinositide Phospholipase C
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Saccharomyces cerevisiae Proteins
MH  - *Transcription Factors
MH  - Type C Phospholipases/pharmacology
EDAT- 1999/08/26 00:00
MHDA- 1999/08/26 00:01
CRDT- 1999/08/26 00:00
PHST- 1999/08/26 00:00 [pubmed]
PHST- 1999/08/26 00:01 [medline]
PHST- 1999/08/26 00:00 [entrez]
AID - 10.1165/ajrcmb.21.3.3638 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1999 Sep;21(3):337-46. doi: 10.1165/ajrcmb.21.3.3638.