PMID- 10460589
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20090929
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 106
IP  - 2
DP  - 1999 Aug
TI  - Def-2, -3, -6 and -8, novel mouse genes differentially expressed in the
      haemopoietic system.
PG  - 335-44
AB  - To identify developmentally regulated genes during myeloid differentiation, a
      self-inactivating retroviral gene-trap vector carrying a
      beta-galactosidase-neomycin (SA/lacZ/neo) fusion gene was constructed and used to
      infect myeloid progenitor cells (FDCP-Mix A4). G418-resistant and
      beta-galactosidase positive cell lines (gene-trap integration [GTI] clones) were 
      established and induced to differentiate in vitro into either macrophages or
      granulocytes. Expression of the trapped loci was monitored at a single-cell level
      by analysing the mature cell types for beta-galactosidase activity. All 37 GTI
      clones tested showed down-regulation either during granulocyte or both
      granulocytic and macrophage differentiation. The endogenous coding regions fused 
      to the SA/lacZ/neo reporter gene were isolated from eight clones. Molecular
      analysis revealed that half of them represented novel mouse genes (def-2, -3, -6 
      and -8) which we confirmed to be differentially expressed in primary haemopoietic
      tissues. Database searches revealed no significant similarities for def-2
      (associated with haemopoietic progenitors) and def-8 (expressed most strongly in 
      peripheral leucocytes). Def-6, which is down-regulated upon the differentiation
      into myeloid as well as erythroid lineages, was found to be closely related but
      not identical with the recently described B-cell-specific switch recombinase
      SWAP-70. Def-3, which is down-regulated upon differentiation into granulocytes
      but expressed in progenitor cells and macrophages, defines a novel family of RNA 
      binding proteins.
FAU - Hotfilder, M
AU  - Hotfilder M
AD  - Max-Delbruck-Laboratory in the MPG, Cologne, Germany.
FAU - Baxendale, S
AU  - Baxendale S
FAU - Cross, M A
AU  - Cross MA
FAU - Sablitzky, F
AU  - Sablitzky F
LA  - eng
SI  - GENBANK/AJ006486
SI  - GENBANK/X96699
SI  - GENBANK/X96700
SI  - GENBANK/X96701
SI  - GENBANK/X96702
SI  - GENBANK/X96703
SI  - GENBANK/X96704
SI  - GENBANK/X96705
SI  - GENBANK/X96706
SI  - GENBANK/X96707
SI  - GENBANK/X96708
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation
MH  - Genetic Vectors
MH  - Hematopoiesis/*genetics
MH  - Hematopoietic System/cytology/metabolism
MH  - Mice
MH  - Stem Cells/cytology/enzymology
MH  - Virus Integration
MH  - beta-Galactosidase/metabolism
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - bjh1551 [pii]
PST - ppublish
SO  - Br J Haematol. 1999 Aug;106(2):335-44.