PMID- 10460412
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20111028
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 108
IP  - 4
DP  - 1999 Aug
TI  - Identification and characterization of MmORC4 and MmORC5, two subunits of the
      mouse origin of replication recognition complex.
PG  - 243-9
AB  - Two new members of the mouse origin recognition complex (ORC) have been cloned
      that are closely related to Saccharomyces cerevisiae ORC4 and ORC5 as well as to 
      their human homolog. Both MmORC4p and MmORC5p have a putative nucleotide
      triphosphate binding motif. Transcription of MmORC4 and MmORC5 is not suppressed 
      in mouse NIH3T3 fibroblasts made quiescent by serum starvation. The transcription
      levels of both ORC genes are constantly high in all phases of the cell cycle. A
      screen based on the two-hybrid approach suggests that the product of the ORC4
      gene interacts with the ORC2, but not with the ORC1 protein. The conservation of 
      structure among members of the ORC4- and ORC5-related family of proteins suggests
      that these proteins play a key role in the initiation of DNA replication in all
      eukaryotes.
FAU - Springer, J
AU  - Springer J
AD  - Institute of Biochemistry Biozentrum, University of Wurzburg, D-97074 Wurzburg,
      Germany.
FAU - Kneissl, M
AU  - Kneissl M
FAU - Putter, V
AU  - Putter V
FAU - Grummt, F
AU  - Grummt F
LA  - eng
SI  - GENBANK/AJ003140
SI  - GENBANK/AJ007360
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (ORC4 protein, human)
RN  - 0 (ORC5 protein, mouse)
RN  - 0 (Orc4 protein, mouse)
RN  - 0 (Origin Recognition Complex)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cell Cycle Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Origin Recognition Complex
MH  - Sequence Homology, Amino Acid
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 91080243.412 [pii]
PST - ppublish
SO  - Chromosoma. 1999 Aug;108(4):243-9.