PMID- 10460410
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20211203
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 108
IP  - 4
DP  - 1999 Aug
TI  - Localization and phosphorylation of HP1 proteins during the cell cycle in
      mammalian cells.
PG  - 220-34
AB  - Mammalian heterochromatin proteins 1 (HP1alpha, HP1beta, and HP1gamma) are
      nonhistone proteins that interact in vitro with a set of proteins that play a
      role in chromatin silencing, transcription, and chromatin remodeling. Using
      antibodies specific for each HP1 isoform, we showed that they segregate in
      distinct nuclear domains of human HeLa cells. By contrast, in mouse 3T3
      interphase cells, HP1alpha and HP1beta are strictly colocalized. In mitotic HeLa 
      cells, all of HP1alpha and a fraction of HP1beta and HP1gamma remain associated
      with chromosomes. Immunostaining of spread HeLa chromosomes showed that HP1alpha 
      is mainly localized on centromeres as shown previously for HP1beta, while
      HP1gamma is distributed on discrete sites on the arms of chromosomes. Biochemical
      analysis showed that HP1alpha and HP1gamma are phosphorylated throughout the cell
      cycle, although more extensively in mitosis than in interphase, while HP1beta
      apparently remains unphosphorylated. Therefore, despite their extensive sequence 
      conservation, mammalian HP1 isoforms differ widely in their nuclear localization,
      mitotic distribution and cell cycle-related phosphorylation. Thus, subtle
      differences in primary sequence and in posttranslational modifications may
      promote their targeting at different chromatin sites, generating pleiotropic
      effects.
FAU - Minc, E
AU  - Minc E
AD  - Departement de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universite
      Paris 7, Paris, France. minc@ijm.jussieu.fr
FAU - Allory, Y
AU  - Allory Y
FAU - Worman, H J
AU  - Worman HJ
FAU - Courvalin, J C
AU  - Courvalin JC
FAU - Buendia, B
AU  - Buendia B
LA  - eng
GR  - CA66974/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Antibodies)
RN  - 0 (CBX1 protein, human)
RN  - 0 (CBX3 protein, human)
RN  - 0 (CBX5 protein, human)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Protein Isoforms)
RN  - 107283-02-3 (Chromobox Protein Homolog 5)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies/chemistry
MH  - *Cell Cycle
MH  - Centromere/metabolism
MH  - Chromobox Protein Homolog 5
MH  - Chromosomal Proteins, Non-Histone/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Isoforms/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Spliceosomes/metabolism
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 91080220.412 [pii]
AID - 10.1007/s004120050372 [doi]
PST - ppublish
SO  - Chromosoma. 1999 Aug;108(4):220-34. doi: 10.1007/s004120050372.