PMID- 10460249
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 17
DP  - 1999 Sep 1
TI  - Specificity and sensitivity of a human olfactory receptor functionally expressed 
      in human embryonic kidney 293 cells and Xenopus Laevis oocytes.
PG  - 7426-33
AB  - Here, we provide the first evidence for functional expression of a human
      olfactory receptor protein (OR17-40) and show that recombinant olfactory
      receptors can be functionally expressed in heterologous systems. A mixture of 100
      different odorants (Henkel 100) elicited a transient increase in intracellular
      [Ca(2+)] in human embryonic kidney 293 (HEK293) cells stably or transiently
      transfected with the plasmid pOR17-40. By subdividing the odorant mixture into
      progressively smaller groups, we identified a single component that represented
      the only effective substance: helional. Only the structurally closely related
      molecule heliotroplyacetone also activated the receptor. Other compounds,
      including piperonal, safrole, and vanillin, were completely ineffective.
      Mock-transfected cells and cells transfected with other receptors showed no
      change in intracellular [Ca(2+)] in response to odor stimulation. We were also
      able to functionally express OR17-40 in Xenopus laevis oocytes. Coexpression of a
      "reporter" channel allowed measurement of the response of oocytes injected with
      the cRNA of the human receptor to the odor mixture Henkel 100. The effective
      substances were the same (helional, heliotropylacetone) as those identified by
      functionally expressing the receptor in HEK293 cells and were active at the same,
      lower micromolar concentration. These findings open the possibility of now
      characterizing the sensitivity and specificity of many, if not all, of the
      hundreds of different human olfactory receptors.
FAU - Wetzel, C H
AU  - Wetzel CH
AD  - Lehrstuhl fur Zellphysiologie, Ruhr-Universitat Bochum, D-44780 Bochum, Germany.
FAU - Oles, M
AU  - Oles M
FAU - Wellerdieck, C
AU  - Wellerdieck C
FAU - Kuczkowiak, M
AU  - Kuczkowiak M
FAU - Gisselmann, G
AU  - Gisselmann G
FAU - Hatt, H
AU  - Hatt H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Complementary)
RN  - 0 (Receptors, Odorant)
RN  - 0 (Recombinant Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Cell Membrane/physiology
MH  - DNA, Complementary
MH  - Female
MH  - Humans
MH  - Kidney
MH  - Membrane Potentials/drug effects
MH  - Models, Neurological
MH  - Molecular Sequence Data
MH  - Odorants
MH  - Oocytes/physiology
MH  - Protein Structure, Secondary
MH  - RNA, Complementary/metabolism
MH  - Receptors, Odorant/chemistry/genetics/*physiology
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Restriction Mapping
MH  - Transfection
MH  - Xenopus laevis
PMC - PMC6782526
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Sep 1;19(17):7426-33.