PMID- 10460238
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 17
DP  - 1999 Sep 1
TI  - A neuronal-specific mammalian homolog of the Drosophila retinal degeneration B
      gene with expression restricted to the retina and dentate gyrus.
PG  - 7317-25
AB  - Mutations in the Drosophila retinal degeneration B (rdgB) gene cause a rapid loss
      of the electrophysiological light response and subsequent light-enhanced
      photoreceptor degeneration. The rdgB gene encodes a protein with an N-terminal
      phosphatidylinositol transfer protein domain, a large C-terminal segment, and
      several hydrophobic regions thought to multiply span the subrhabdomeric cisternal
      membrane. A mammalian rdgB homolog (m-rdgB1) was previously identified and shown 
      to exhibit widespread tissue distribution and functionally rescue the Drosophila 
      rdgB mutant phenotypes. We describe a second mammalian rdgB homolog (m-rdgB2)
      that possesses 46% amino acid identity to Drosophila RdgB and 56% identity to
      M-RdgB1. M-RdgB2 possesses a neuronal-specific expression pattern, with high
      levels in the retina and the dentate gyrus mossy fibers and dendritic field.
      Using M-RdgB2-specific antibodies and subcellular fractionation, we demonstrate
      that M-RdgB2 is not an integral membrane protein but is stably associated with a 
      particulate fraction through protein-protein interactions. Although transgenic
      expression of M-RdgB2 in rdgB2 null mutant flies suppressed the retinal
      degeneration, it failed to fully restore the electrophysiological light response.
      Because transgenic expression of M-RdgB2 does not restore the wild-type phenotype
      to rdgB2 mutant flies to the same extent as M-RdgB1, functional differences
      likely exist between the two M-RdgB homologs.
FAU - Lu, C
AU  - Lu C
AD  - Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical
      School, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA.
FAU - Vihtelic, T S
AU  - Vihtelic TS
FAU - Hyde, D R
AU  - Hyde DR
FAU - Li, T
AU  - Li T
LA  - eng
SI  - GENBANK/AF058693
GR  - EY08058/EY/NEI NIH HHS/United States
GR  - EY10309/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Drosophila Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Pitpnm2 protein, mouse)
RN  - 0 (Recombinant Proteins)
RN  - 139135-48-1 (rdgB protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/*metabolism
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Crosses, Genetic
MH  - Dentate Gyrus/*metabolism
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/*genetics
MH  - Exons
MH  - Eye Proteins/biosynthesis/chemistry/*genetics
MH  - Introns
MH  - Mammals
MH  - Membrane Proteins/chemistry/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Myocardium/metabolism
MH  - Organ Specificity
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Retina/*metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
PMC - PMC6782490
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Sep 1;19(17):7317-25.