PMID- 10460162
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20141120
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 34
DP  - 1999 Aug 24
TI  - The protein Z-dependent protease inhibitor is a serpin.
PG  - 11073-8
AB  - In the presence of phospholipid vesicles and calcium ions, protein Z (PZ) serves 
      as a cofactor for the inhibition of coagulation factor Xa by a plasma protein
      called PZ-dependent protease inhibitor (ZPI). To further characterize ZPI, its
      cDNA has been isolated and cloned from a human liver cDNA library. The ZPI cDNA
      is 2.44 kb in length and has a relatively long 5' region (466 nt) that contains
      six potential ATG translation start codons. ATG's 1-4 are followed by short open 
      reading frames, whereas ATG(5) and ATG(6) are in an uninterrupted open reading
      frame that includes the encoded ZPI protein. In vitro experiments show that
      ATG(6) is sufficient for the expression of rZPI in cultured Chinese hamster ovary
      cells. Northern analysis suggests the liver is a major site of ZPI synthesis. The
      predicted 423 residue amino acid sequence of the mature ZPI protein is 25-35%
      homologous with members of the serpin superfamily of protease inhibitors and is
      78% identical to the amino acid sequence predicted by a previously described cDNA
      isolated from rat liver, regeneration-associated serpin protein-1 (rasp-1). Thus,
      ZPI is likely the human homologue of rat rasp-1. Alignment of the amino acid
      sequence of ZPI with those of other serpins predicts that Y387 is the P(1)
      residue at the reactive center of the ZPI molecule. Consistent with this notion, 
      rZPI(Y387A), an altered form of ZPI in which tyrosine 387 has been changed to
      alanine, lacks PZ-dependent factor Xa inhibitory activity.
FAU - Han, X
AU  - Han X
AD  - Division of Hematology, Barnes-Jewish Hospital at Washington University School of
      Medicine, St. Louis, Missouri 63110, USA.
FAU - Huang, Z F
AU  - Huang ZF
FAU - Fiehler, R
AU  - Fiehler R
FAU - Broze, G J Jr
AU  - Broze GJ Jr
LA  - eng
SI  - GENBANK/AF181467
GR  - HL-60782/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Blood Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Factor Xa Inhibitors)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Serpins)
RN  - 0 (plasma protein Z)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blood Coagulation Tests
MH  - Blood Proteins/*physiology
MH  - CHO Cells
MH  - Cricetinae
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Factor Xa Inhibitors
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protease Inhibitors/*chemistry/isolation & purification
MH  - Rabbits
MH  - Rats
MH  - Recombinant Proteins/biosynthesis/genetics
MH  - Serpins/*chemistry/genetics/isolation & purification
EDAT- 1999/08/25 09:00
MHDA- 2001/03/28 10:01
CRDT- 1999/08/25 09:00
PHST- 1999/08/25 09:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/08/25 09:00 [entrez]
AID - 10.1021/bi990641a [doi]
AID - bi990641a [pii]
PST - ppublish
SO  - Biochemistry. 1999 Aug 24;38(34):11073-8. doi: 10.1021/bi990641a.