PMID- 10460157
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20071114
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 34
DP  - 1999 Aug 24
TI  - Replication protein A stimulates proliferating cell nuclear antigen-dependent
      repair of abasic sites in DNA by human cell extracts.
PG  - 11021-5
AB  - Base excision repair (BER) pathway is the major cellular process for removal of
      endogenous base lesions and apurinic/apyrimidinic (AP) sites in DNA. There are
      two base excision repair subpathways in mammalian cells, characterized by the
      number of nucleotides synthesized into the excision patch. They are the
      "single-nucleotide" (one nucleotide incorporated) and the "long-patch" (several
      nucleotides incorporated) BER pathways. Proliferating cell nuclear antigen (PCNA)
      is known to be an essential factor in long-patch base excision repair. We have
      studied the role of replication protein A (RPA) in PCNA-dependent, long-patch BER
      of AP sites in human cell extracts. PCNA and RPA were separated from the other
      BER proteins by fractionation of human whole-cell extract on a phosphocellulose
      column. The protein fraction PC-FII (phosphocellulose fraction II), which does
      not contain RPA and PCNA but otherwise contains all core BER proteins required
      for PCNA-dependent BER (AP endonuclease, DNA polymerases delta, beta and DNA
      ligase, and FEN1 endonuclease), had reduced ability to repair plasmid DNA
      containing AP sites. Purified PCNA or RPA, when added separately, could only
      partially restore the PC-FII repair activity of AP sites. However, additions of
      both proteins together greatly stimulated AP site repair by PC-FII. These results
      demonstrate a role for RPA in PCNA-dependent BER of AP sites.
FAU - Dianov, G L
AU  - Dianov GL
AD  - Laboratory of Molecular Genetics, National Institute on Aging, National
      Institutes of Health, Baltimore, Maryland 21224, USA. dianovg@grc.nia.nih.gov
FAU - Jensen, B R
AU  - Jensen BR
FAU - Kenny, M K
AU  - Kenny MK
FAU - Bohr, V A
AU  - Bohr VA
LA  - eng
GR  - CA71612/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Cation Exchange Resins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RPA1 protein, human)
RN  - 0 (Replication Protein A)
RN  - 9004-34-6 (Cellulose)
RN  - 9007-49-2 (DNA)
RN  - 9015-14-9 (phosphocellulose)
RN  - EC 3.1.21.2 (Deoxyribonuclease IV (Phage T4-Induced))
RN  - EC 4.2.- (Carbon-Oxygen Lyases)
RN  - EC 4.2.99.18 (DNA-(Apurinic or Apyrimidinic Site) Lyase)
SB  - IM
MH  - Base Sequence
MH  - Carbon-Oxygen Lyases/*metabolism
MH  - Cation Exchange Resins
MH  - Cell Fractionation
MH  - Cellulose/analogs & derivatives
MH  - Chromatography, Gel
MH  - DNA/*metabolism
MH  - *DNA Repair
MH  - DNA-(Apurinic or Apyrimidinic Site) Lyase
MH  - DNA-Binding Proteins/isolation & purification/*physiology
MH  - Deoxyribonuclease IV (Phage T4-Induced)
MH  - Drug Synergism
MH  - Humans
MH  - Lymphocytes/enzymology/*metabolism
MH  - Molecular Sequence Data
MH  - Proliferating Cell Nuclear Antigen/isolation & purification/*physiology
MH  - Replication Protein A
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 10.1021/bi9908890 [doi]
AID - bi9908890 [pii]
PST - ppublish
SO  - Biochemistry. 1999 Aug 24;38(34):11021-5. doi: 10.1021/bi9908890.