PMID- 10458916
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20131121
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 60
IP  - 1
DP  - 1999 Aug 15
TI  - Cloning and characterization of ZNF236, a glucose-regulated Kruppel-like
      zinc-finger gene mapping to human chromosome 18q22-q23.
PG  - 105-9
AB  - We report the cDNA cloning and characterization of ZNF236, a novel Kruppel-like
      zinc-finger gene initially identified by its glucose-regulated expression in
      human mesangial cells using mRNA differential display. Using the differential
      display fragment as a probe, we screened a human fetal kidney cDNA library and
      isolated several clones representing two differently spliced mRNA transcripts,
      designated ZNF236a and -b. Both transcripts were identical apart from the
      presence of an additional exon in ZNF236a that truncates the open reading frame. 
      RT-PCR analysis confirmed the expression of both transcripts to be upregulated in
      human mesangial cells in response to elevated levels of d-glucose. ZNF236a and -b
      cDNAs encode polypeptides of 174 and 204 kDa, containing 25 and 30 C(2)H(2)
      zinc-finger motifs, respectively. Northern blot analysis showed that ZNF236 is
      ubiquitously expressed in all human tissues tested. Expression levels were
      highest in skeletal muscle and brain, intermediate in heart, pancreas, and
      placenta, and lowest in kidney, liver, and lung. Southern zoo blot analysis
      indicated that ZNF236 is conserved in the genomes of all mammalian species
      tested, but not in yeast. The mapping of ZNF236 to human chromosome 18q22-q23,
      close to the IDDM6 locus, coupled with the glucose-regulated expression of the
      gene in human mesangial cells, suggests that ZNF236 may be a candidate gene for
      diabetic nephropathy.
CI  - Copyright 1999 Academic Press.
FAU - Holmes, D I
AU  - Holmes DI
AD  - Division of Biomedical Sciences, Imperial College School of Medicine, BMS
      Building, South Kensington, London, SW7 2AZ, United Kingdom.
FAU - Wahab, N A
AU  - Wahab NA
FAU - Mason, R M
AU  - Mason RM
LA  - eng
SI  - GENBANK/AF085243
SI  - GENBANK/AF085244
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ZNF236 protein, human)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Carrier Proteins/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics/isolation & purification
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - Glucose/physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kruppel-Like Transcription Factors
MH  - Molecular Sequence Data
MH  - Protein Isoforms/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - *Repressor Proteins
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Transcription Factors/*genetics
MH  - Zinc Fingers/*genetics
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 10.1006/geno.1999.5897 [doi]
AID - S0888-7543(99)95897-4 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 15;60(1):105-9. doi: 10.1006/geno.1999.5897.