PMID- 10458915
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20081016
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 60
IP  - 1
DP  - 1999 Aug 15
TI  - Identification, chromosomal assignment, and expression analysis of the human
      homeodomain-containing gene Orthopedia (OTP).
PG  - 96-104
AB  - Homeodomain (HD) genes are helix-turn-helix transcription factors that play key
      roles in the specification of cell fates. In the central nervous system (CNS), HD
      genes not only position cells along an axis, but also specify cell migration
      patterns and may influence axonal connectivity. In an effort to identify novel HD
      genes involved in the development of the human CNS, we have cloned,
      characterized, and mapped the human homologue of the murine HD gene Orthopedia
      (Otp), whose product is found in multiple cell groups within the mouse
      hypothalamus, amygdala, and brain stem. Human cDNA and genomic libraries were
      screened with probes derived from mouse Otp sequences to find the human
      homologue, OTP. The deduced amino acid sequence of the open reading frame of the 
      human cDNA is 99% homologous to mouse Otp and demonstrates a high degree of
      conservation when compared to sea urchin and Drosophila. OTP was mapped to human 
      chromosome 5q13.3 using radiation hybrid panel mapping and fluorescence in situ
      hybridization. Flanking markers were identified from YAC clones containing OTP. A
      single putative OTP gene product was found in 17-week human fetal brain tissue by
      Western blot analysis using a novel polyclonal antibody raised against a
      conserved 13-amino-acid sequence at the C-terminus of the OTP protein. Expression
      in the developing human hypothalamus was confirmed by immunohistochemistry.
CI  - Copyright 1999 Academic Press.
FAU - Lin, X
AU  - Lin X
AD  - Child Study Center, Yale University School of Medicine, New Haven, Connecticut,
      06520-7900, USA.
FAU - State, M W
AU  - State MW
FAU - Vaccarino, F M
AU  - Vaccarino FM
FAU - Greally, J
AU  - Greally J
FAU - Hass, M
AU  - Hass M
FAU - Leckman, J F
AU  - Leckman JF
LA  - eng
GR  - MH49351/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (OTP protein, human)
RN  - 0 (otp protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/embryology/metabolism
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 5/genetics
MH  - DNA, Complementary/chemistry/genetics
MH  - *Drosophila Proteins
MH  - Exons
MH  - Gene Expression
MH  - Gene Expression Regulation, Developmental
MH  - Genes/genetics
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 10.1006/geno.1999.5882 [doi]
AID - S0888-7543(99)95882-2 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 15;60(1):96-104. doi: 10.1006/geno.1999.5882.