PMID- 10458914
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20161124
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 60
IP  - 1
DP  - 1999 Aug 15
TI  - Cloning, expression profile, and genomic organization of the mouse STAP/A170
      gene.
PG  - 87-95
AB  - The preferential screening of cDNA libraries derived from the mouse osteoblastic 
      cell line MC3T3-E1 has yielded a cDNA clone encoding a 442-amino-acid protein
      designated STAP (signal transduction and adaptor protein), which contains several
      motifs shared among transcription factors and adaptors such as a Zn-finger like
      motif, a proline-rich domain, and a PEST sequence. The amino acid sequence
      homology search also reveals that STAP is identical to a mouse oxidative stress
      protein, A170, and has 90% homology with a human p62 protein that binds to the
      tyrosine kinase p56(lck) SH2 domain. Northern blot analysis indicated a broad
      expression profile of STAP mRNA in various tissues and cell lines. In MC3T3-E1
      cells, STAP mRNA was induced by treatment with TGF-beta, but not with BMP-2 or
      GDF-5. Analysis of the mouse STAP gene isolated from the genomic library revealed
      that the STAP gene spans a region of over 11 kb and comprises eight exons. The
      transcription start site was identified by primer extension analysis to be
      located 35 bp upstream from the translation initiation site. Sequencing analysis 
      of the 5' flanking region of the STAP gene revealed multiple consensus
      motifs/sequences for several DNA binding transcription factors. The STAP gene had
      a TATA box, but no CCAAT box. Potential Sp1, AP-1, NF-E2, MyoD, and NF-kappaB
      binding sites were found in the 5' flanking region (1.4 kb) of the STAP gene.
CI  - Copyright 1999 Academic Press.
FAU - Okazaki, M
AU  - Okazaki M
AD  - Discovery Research Laboratories, Hoechst Marion Roussel Ltd., Kawagoe, 350-1162, 
      Japan.
FAU - Ito, S
AU  - Ito S
FAU - Kawakita, K
AU  - Kawakita K
FAU - Takeshita, S
AU  - Takeshita S
FAU - Kawai, S
AU  - Kawai S
FAU - Makishima, F
AU  - Makishima F
FAU - Oda, H
AU  - Oda H
FAU - Kakinuma, A
AU  - Kakinuma A
LA  - eng
SI  - GENBANK/E08146
PT  - Journal Article
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (DNA, Complementary)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sequestosome-1 Protein)
RN  - 0 (Sqstm1 protein, mouse)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - 3T3 Cells
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - DNA/chemistry/genetics
MH  - DNA, Complementary/chemistry/genetics
MH  - Exons
MH  - Gene Expression
MH  - Genes/*genetics
MH  - Heat-Shock Proteins/*genetics
MH  - Introns
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequestosome-1 Protein
MH  - TATA Box
MH  - Tissue Distribution
MH  - Transcription, Genetic
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 10.1006/geno.1999.5902 [doi]
AID - S0888-7543(99)95902-5 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 15;60(1):87-95. doi: 10.1006/geno.1999.5902.