PMID- 10458913
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 60
IP  - 1
DP  - 1999 Aug 15
TI  - cDNA isolation, genomic structure, regulation, and chromosomal localization of
      human lung Kruppel-like factor.
PG  - 78-86
AB  - Lung Kruppel-like factor (LKLF) is a zinc finger transcription factor critical
      for embryonic development. We have previously identified and isolated the mouse
      LKLF gene and examined its role using gene targeting. In this report, we describe
      the isolation and molecular characterization of the human homolog of murine LKLF.
      The human and mouse LKLF homologs exhibit an 85% nucleotide identity and share
      90% amino acid similarity. Furthermore, the 5' sequence in the proximal promoter 
      region and 3' untranslated region are also conserved between the two species. Of 
      particular interest is the finding that while sequences in the proximal promoter 
      have diverged between mouse and human, a region of 75 nucleotides is essentially 
      identical. Site-directed mutagenesis in this region impairs the ability of the
      LKLF promoter to drive reporter gene expression, indicating that it represents a 
      novel transcriptional element important in the regulation of LKLF gene
      expression. The activation domain is highly proline-rich and, similar to mouse
      LKLF, contains 22% proline residues. The human LKLF transcriptional unit is
      located in a genomic region of approximately 3 kb on chromosome 19p13.1. This
      region of chromosome 19 is known to contain genes involved in various human
      diseases. Like mouse LKLF, human LKLF consists of three exons that are
      interrupted by two small introns. The locations of intron/exon boundaries and
      splice sites are conserved between two homologs. Northern analysis shows that
      LKLF is expressed in lung in addition to heart, skeletal muscle, placenta, and
      pancreas. The isolation and chromosomal mapping of human LKLF will make it
      possible to initiate studies devoted to assess the involvement of this gene in
      human disease(s).
CI  - Copyright 1999 Academic Press.
FAU - Wani, M A
AU  - Wani MA
AD  - College of Medicine, University of Cincinnati, Cincinnati, Ohio, 45267-0524, USA.
FAU - Conkright, M D
AU  - Conkright MD
FAU - Jeffries, S
AU  - Jeffries S
FAU - Hughes, M J
AU  - Hughes MJ
FAU - Lingrel, J B
AU  - Lingrel JB
LA  - eng
SI  - GENBANK/AF134053
GR  - HL 57281/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (KLF2 protein, human)
RN  - 0 (Klf2 protein, mouse)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Trans-Activators)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 19/genetics
MH  - Cloning, Molecular
MH  - DNA/chemistry/*genetics/isolation & purification
MH  - DNA, Complementary/chemistry/*genetics/isolation & purification
MH  - Female
MH  - Gene Expression
MH  - Genes/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kruppel-Like Transcription Factors
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Tissue Distribution
MH  - Trans-Activators/*genetics
MH  - Zinc Fingers/genetics
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 10.1006/geno.1999.5888 [doi]
AID - S0888-7543(99)95888-3 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 15;60(1):78-86. doi: 10.1006/geno.1999.5888.