PMID- 10458907
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 60
IP  - 1
DP  - 1999 Aug 15
TI  - Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta.
PG  - 20-30
AB  - The Saccharomyces cerevisiae RAD30 gene encodes a novel eukaryotic DNA
      polymerase, pol eta that is able to replicate across cis-syn cyclobutane
      pyrimidine dimers both accurately and efficiently. Very recently, a human homolog
      of RAD30 was identified, mutations in which result in the sunlight-sensitive,
      cancer-prone, Xeroderma pigmentosum variant group phenotype. We report here the
      cloning and localization of a second human homolog of RAD30. Interestingly,
      RAD30B is localized on chromosome 18q21.1 in a region that is often implicated in
      the etiology of many human cancers. The mouse homolog (Rad30b) is located on
      chromosome 18E2. The human RAD30B and mouse Rad30b mRNA transcripts, like many
      repair proteins, are highly expressed in the testis. In situ hybridization
      analysis indicates that expression of mouse Rad30b occurs predominantly in
      postmeiotic round spermatids. Database searches revealed genomic and EST
      sequences from other eukaryotes such as Aspergillus nidulans, Schizosaccharomyces
      pombe, Brugia malayi, Caenorhabditis elegans, Trypanosoma cruzi, Arabidopsis
      thaliana, and Drosophila melanogaster that also encode putative homologs of
      RAD30, thereby suggesting that Rad30-dependent translesion DNA synthesis is
      conserved within the eukaryotic kingdom.
CI  - Copyright 1999 Academic Press.
FAU - McDonald, J P
AU  - McDonald JP
AD  - Section on DNA Replication, Repair and Mutagenesis, National Institute of Child
      Health and Human Development, Bethesda, Maryland, 20892-2725, USA.
FAU - Rapic-Otrin, V
AU  - Rapic-Otrin V
FAU - Epstein, J A
AU  - Epstein JA
FAU - Broughton, B C
AU  - Broughton BC
FAU - Wang, X
AU  - Wang X
FAU - Lehmann, A R
AU  - Lehmann AR
FAU - Wolgemuth, D J
AU  - Wolgemuth DJ
FAU - Woodgate, R
AU  - Woodgate R
LA  - eng
SI  - GENBANK/AF140501
SI  - GENBANK/AF151691
GR  - R01HD34915/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DinB protein, E coli)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 98059-80-4 (UmuC protein, E coli)
RN  - EC 2.7.7.- (DNA polymerase iota)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
RN  - EC 2.7.7.- (REV1 protein, S cerevisiae)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - EC 2.7.7.7 (Rad30 protein)
SB  - IM
SB  - S
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Proteins/genetics
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Chromosomes/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - DNA, Complementary/chemistry/genetics/isolation & purification
MH  - DNA-Directed DNA Polymerase/*genetics
MH  - *Escherichia coli Proteins
MH  - Evolution, Molecular
MH  - Fungal Proteins/genetics
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Nucleotidyltransferases
MH  - Phylogeny
MH  - Saccharomyces cerevisiae/*genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Testis/metabolism
OTO - NASA
OT  - Non-programmatic
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 10.1006/geno.1999.5906 [doi]
AID - S0888-7543(99)95906-2 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 15;60(1):20-30. doi: 10.1006/geno.1999.5906.