PMID- 10458905
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 60
IP  - 1
DP  - 1999 Aug 15
TI  - Sequence-ready contig for the 1.4-cM ductal carcinoma in situ loss of
      heterozygosity region on chromosome 8p22-p23.
PG  - 1-11
AB  - We report the construction of an approximately 1.7-Mb sequence-ready YAC/BAC
      clone contig of 8p22-p23. This chromosomal region has been associated with
      frequent loss of heterozygosity (LOH) in breast, ovarian, prostate, head and
      neck, and liver cancer. We first constructed a meiotic linkage map for 8p to
      resolve previously reported conflicting map orders from the literature. The
      target region containing a putative tumor suppressor gene was defined by
      allelotyping 65 cases of sporadic ductal carcinoma in situ with 18 polymorphic
      markers from 8p. The minimal region of loss encompassed the interval between
      D8S520 and D8S261, and one tumor had loss of D8S550 only. We chose to begin
      physical mapping of this minimal LOH region by concentrating on the distal end,
      which includes D8S550. A fine-structure radiation hybrid map for the region that 
      extends from D8S520 (distal) to D8S1759 (proximal) was prepared, followed by
      construction of a single, integrated YAC/BAC contig for the interval. The
      approximately 1730-kb contig consists of 13 YACs and 27 BACs. Fifty-four
      sequence-tagged sites (STSs) developed from BAC insert end-sequences and 11
      expressed sequence tags were localized within the contig by STS content mapping. 
      In addition, four unique cDNA clones from the region were isolated and fully
      sequenced. This integrated YAC/BAC resource provides the starting point for
      transcription mapping, genomic sequencing, and positional cloning of this region.
CI  - Copyright 1999 Academic Press.
FAU - Wang, J C
AU  - Wang JC
AD  - Division of Human Molecular Genetics, Washington University School of Medicine,
      St. Louis, Missouri, 63110, USA.
FAU - Radford, D M
AU  - Radford DM
FAU - Holt, M S
AU  - Holt MS
FAU - Helms, C
AU  - Helms C
FAU - Goate, A
AU  - Goate A
FAU - Brandt, W
AU  - Brandt W
FAU - Parik, M
AU  - Parik M
FAU - Phillips, N J
AU  - Phillips NJ
FAU - DeSchryver, K
AU  - DeSchryver K
FAU - Schuh, M E
AU  - Schuh ME
FAU - Fair, K L
AU  - Fair KL
FAU - Ritter, J H
AU  - Ritter JH
FAU - Marshall, P
AU  - Marshall P
FAU - Donis-Keller, H
AU  - Donis-Keller H
LA  - eng
SI  - GENBANK/AF122926
SI  - GENBANK/AF122927
SI  - GENBANK/AF122928
SI  - GENBANK/AF122929
SI  - GENBANK/AF122930
SI  - GENBANK/AF122931
SI  - GENBANK/AF122932
SI  - GENBANK/AF122933
SI  - GENBANK/AF122934
SI  - GENBANK/AF122935
SI  - GENBANK/AF122936
SI  - GENBANK/AF122937
SI  - GENBANK/AF122938
SI  - GENBANK/AF122939
SI  - GENBANK/AF122940
SI  - GENBANK/AF122941
SI  - GENBANK/AF122942
SI  - GENBANK/AF122943
SI  - GENBANK/AF122944
SI  - GENBANK/AF122945
SI  - GENBANK/AF122946
SI  - GENBANK/AF122947
SI  - GENBANK/AF122948
SI  - GENBANK/AF124266
SI  - GENBANK/AF124267
SI  - GENBANK/AF124268
SI  - GENBANK/AF124269
SI  - GENBANK/AF124270
SI  - GENBANK/AF124271
SI  - GENBANK/AF124272
GR  - HG00469/HG/NHGRI NIH HHS/United States
GR  - HG01066/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Carcinoma in Situ/*genetics
MH  - Carcinoma, Ductal, Breast/*genetics
MH  - Chromosome Walking
MH  - Chromosomes, Bacterial/genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Contig Mapping
MH  - DNA, Neoplasm/chemistry/genetics
MH  - Expressed Sequence Tags
MH  - Female
MH  - Gene Library
MH  - Genes, Tumor Suppressor/genetics
MH  - Humans
MH  - Hybrid Cells
MH  - *Loss of Heterozygosity
MH  - Microsatellite Repeats
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA
MH  - Sequence Tagged Sites
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 10.1006/geno.1999.5905 [doi]
AID - S0888-7543(99)95905-0 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 15;60(1):1-11. doi: 10.1006/geno.1999.5905.