PMID- 10458606
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 98
IP  - 3
DP  - 1999 Aug 6
TI  - Nuclear trafficking of Cubitus interruptus in the transcriptional regulation of
      Hedgehog target gene expression.
PG  - 305-16
AB  - Transcriptional activation of Hedgehog (Hh) target genes requires Cubitus
      interruptus, a 155 kDa cytoplasmic zinc finger protein (Ci155), which in the
      absence of Hh signaling is processed to form a nuclear repressor (Ci75). We show 
      that Hh signaling reduces phosphorylation of Ci155, and this reduction in turn
      appears to decrease processing. Blocking processing with proteasome inhibitors or
      altered Ci proteins, however, is insufficient for activation of Hh targets. We
      find that Hh signaling increases the rate of Ci155 nuclear import, resulting in
      significant nuclear accumulation. Even in the absence of signaling, nuclear
      accumulation of Ci155 suffices for significant induction of Hh targets, and
      active nuclear export of Ci155 is an essential mechanism for maintenance of the
      unstimulated state.
FAU - Chen, C H
AU  - Chen CH
AD  - Howard Hughes Medical Institute, Department of Molecular Biology and Genetics,
      The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
FAU - von Kessler, D P
AU  - von Kessler DP
FAU - Park, W
AU  - Park W
FAU - Wang, B
AU  - Wang B
FAU - Ma, Y
AU  - Ma Y
FAU - Beachy, P A
AU  - Beachy PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ci protein, Drosophila)
RN  - 149291-21-4 (hh protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - DNA-Binding Proteins/*genetics/*metabolism
MH  - Drosophila/*genetics
MH  - *Drosophila Proteins
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - Hedgehog Proteins
MH  - Insect Proteins/*genetics/metabolism
MH  - Kinetics
MH  - Polymerase Chain Reaction
MH  - Recombinant Proteins/metabolism
MH  - Restriction Mapping
MH  - Signal Transduction
MH  - Transcription Factors
MH  - *Transcription, Genetic
MH  - Transfection
MH  - Zinc Fingers
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - S0092-8674(00)81960-1 [pii]
AID - 10.1016/s0092-8674(00)81960-1 [doi]
PST - ppublish
SO  - Cell. 1999 Aug 6;98(3):305-16. doi: 10.1016/s0092-8674(00)81960-1.