PMID- 10458320
OWN - NLM
STAT- MEDLINE
DCOM- 19991006
LR  - 20191115
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 54
IP  - 1
DP  - 1999 Jul
TI  - Molecular characterization of a novel human natural killer cell receptor
      homologous to mouse 2B4.
PG  - 27-34
AB  - Natural killer (NK) cells spontaneously detect and kill cancerous and virally
      infected cells through receptors that transduce either activating or inhibiting
      signals. The majority of well studied NK receptors are involved in inhibitory
      signaling. However, we have previously described an activating receptor, 2B4,
      expressed on all murine NK cells and a subset of T cells that mediate non-major
      histocompatibility complex (MHC) restricted killing. Anti-2B4 monoclonal
      antibodies directed against IL-2-activated NK cells enhanced their destruction of
      tumor cells. Recently, we determined binding of 2B4 to CD48 with a much higher
      affinity than CD2 to CD48. Here we describe the molecular characterization of a
      cDNA clone homologous to mouse 2B4, isolated from a human NK cell library. The
      cDNA clone contained an open reading frame encoding a polypeptide chain of 365
      amino acid residues. The predicted protein sequence showed 70% similarity to
      murine 2B4. Additionally, it has 48, 45, and 43% similarity to human CD84, CDw150
      (SLAM), and CD48, respectively. RNA blot analysis indicates the presence of 3 kb 
      and 5 kb transcripts in T- and NK-cell lines. A single transcript of 3 kb is
      identified in poly(A)+ RNA from human spleen, peripheral blood leukocytes, and
      lymph node, whereas, the level of expression in bone marrow and fetal liver was
      indeterminate. Preliminary functional data suggests that NK-cell interaction with
      target cells via 2B4 modulates human NK-cell cytolytic activity.
FAU - Boles, K S
AU  - Boles KS
AD  - Department of Molecular Biology and Immunology, University of North Texas Health 
      Science Center, Fort Worth 76107-2699, USA.
FAU - Nakajima, H
AU  - Nakajima H
FAU - Colonna, M
AU  - Colonna M
FAU - Chuang, S S
AU  - Chuang SS
FAU - Stepp, S E
AU  - Stepp SE
FAU - Bennett, M
AU  - Bennett M
FAU - Kumar, V
AU  - Kumar V
FAU - Mathew, P A
AU  - Mathew PA
LA  - eng
GR  - P01 AI 38938/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antigens, CD)
RN  - 0 (CD244 protein, human)
RN  - 0 (Cd244a protein, mouse)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Signaling Lymphocytic Activation Molecule Family)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigens, CD
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Humans
MH  - Killer Cells, Natural/*immunology
MH  - Membrane Glycoproteins/*genetics/immunology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Receptors, Immunologic/*genetics/immunology
MH  - Sequence Homology
MH  - Signaling Lymphocytic Activation Molecule Family
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1034/j.1399-0039.1999.540103.x [doi]
PST - ppublish
SO  - Tissue Antigens. 1999 Jul;54(1):27-34. doi: 10.1034/j.1399-0039.1999.540103.x.