PMID- 10457177
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 11
IP  - 8
DP  - 1999 Aug
TI  - Acidic calponin immunoreactivity in postnatal rat brain and cultures: subcellular
      localization in growth cones, under the plasma membrane and along actin and glial
      filaments.
PG  - 2801-12
AB  - Acidic calponin, an F-actin-binding protein, is particularly enriched in brain,
      where calponin protein and mRNA are mainly expressed by neurons. The presence of 
      calponin immunoreactivity in cultured astroglial cells has been reported, but the
      presence of acidic calponin in astrocytes in vivo appears equivocal. For the
      present study, we raised a specific polyclonal antibody against the 16-residue
      synthetic peptide covering the sequence E311-Q326 (EYPDEYPREYQYGDDQ) situated at 
      the carboxy-terminal end of rat acidic calponin, and we investigated the cellular
      and subcellular localization of the protein in the developing central nervous
      system. Our results show that acidic calponin is particularly enriched in: (i)
      growth cones and submembranous fields of maturing cerebellar and cortical cells, 
      where it codistributes with microfilaments and (ii) glial cells in vivo,
      including radial glia, glia limitans, Bergmann glia and mature astrocytes, and ex
      vivo, where acidic calponin strongly colocalizes with intermediate glial
      fibrillary acidic protein (GFAP) and vimentin filaments. Finally, up to four
      acidic calponin subtypes with different isoelectric point (pI) values were
      identified by two-dimensional gel electrophoresis of cerebellar and hippocampal
      extracts. The more acidic isoforms were developmentally regulated. As only one
      single mRNA for acidic calponin has been identified, these isoforms must reflect 
      postsynthesis changes probably related to the particular functions of acidic
      calponin in maturing cells. Although brain acidic calponin's exact role remains
      uncertain, the present data suggest that it is involved in neuronal and glial
      plasticity.
FAU - Plantier, M
AU  - Plantier M
AD  - Universite Rene Descartes, Paris V and INSERM U29, Paris, France.
FAU - Fattoum, A
AU  - Fattoum A
FAU - Menn, B
AU  - Menn B
FAU - Ben-Ari, Y
AU  - Ben-Ari Y
FAU - Der Terrossian, E
AU  - Der Terrossian E
FAU - Represa, A
AU  - Represa A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Acids)
RN  - 0 (Actins)
RN  - 0 (Biomarkers)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (calponin)
SB  - IM
MH  - Acids/*metabolism
MH  - Actin Cytoskeleton/metabolism
MH  - Actins/metabolism
MH  - Aging/metabolism
MH  - Animals
MH  - Animals, Newborn/*metabolism
MH  - Biomarkers
MH  - Brain/cytology/growth & development/*metabolism
MH  - Calcium-Binding Proteins/*metabolism
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Growth Cones/metabolism
MH  - Immunohistochemistry
MH  - Intermediate Filaments/metabolism
MH  - Microfilament Proteins
MH  - Neuroglia/metabolism
MH  - Rats
MH  - Subcellular Fractions/metabolism
MH  - Tissue Distribution/physiology
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - ejn702 [pii]
AID - 10.1046/j.1460-9568.1999.00702.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 1999 Aug;11(8):2801-12. doi: 10.1046/j.1460-9568.1999.00702.x.