PMID- 10457025
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 126
IP  - 18
DP  - 1999 Sep
TI  - A Drosophila Axin homolog, Daxin, inhibits Wnt signaling.
PG  - 4165-73
AB  - The vertebrate Axin protein, the product of the mouse fused gene, binds to
      beta-catenin to inhibit Wnt signaling. We have identified a homolog of Axin in
      Drosophila, Daxin. Using double-stranded RNA interference, we generated
      loss-of-function phenotypes that are similar to overexpression of the Drosophila 
      Wnt gene wingless (wg). Overexpression of Daxin produces phenotypes similar to
      loss of wg. In addition, we show that Daxin overexpression can modify phenotypes 
      elicited by wg and another Drosophila Wnt gene, DWnt-2. Using immunoprecipitation
      of endogenous Daxin protein from embryos we show that Daxin interacts with
      Armadillo and Zeste-white 3. The loss-of-function and overexpression phenotypes
      show that Daxin, like its mammalian counterpart, acts as a negative regulator of 
      wg/Wnt signaling.
FAU - Willert, K
AU  - Willert K
AD  - Howard Hughes Medical Institute, Department of Developmental Biology, Stanford
      University School of Medicine, Stanford, CA 94305, USA.
FAU - Logan, C Y
AU  - Logan CY
FAU - Arora, A
AU  - Arora A
FAU - Fish, M
AU  - Fish M
FAU - Nusse, R
AU  - Nusse R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (ARM protein, Drosophila)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Armadillo Domain Proteins)
RN  - 0 (Axin Protein)
RN  - 0 (Axn protein, Drosophila)
RN  - 0 (Carrier Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Wnt1 Protein)
RN  - 0 (Wnt2 Protein)
RN  - 0 (Wnt2 protein, Drosophila)
RN  - 0 (wg protein, Drosophila)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Sgg protein, Drosophila)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Armadillo Domain Proteins
MH  - Axin Protein
MH  - Base Sequence
MH  - Carrier Proteins/*genetics/*metabolism
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/embryology/*genetics
MH  - Embryo, Nonmammalian
MH  - Expressed Sequence Tags
MH  - Eye/growth & development
MH  - Eye Abnormalities/genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Genetic Techniques
MH  - *Glycogen Synthase Kinase 3
MH  - Insect Proteins/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Ovary/growth & development/metabolism
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Proteins/genetics
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - RNA/genetics
MH  - *Repressor Proteins
MH  - Sequence Homology, Amino Acid
MH  - *Signal Transduction
MH  - *Trans-Activators
MH  - Transcription Factors
MH  - Wings, Animal/growth & development
MH  - Wnt1 Protein
MH  - Wnt2 Protein
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1242/dev.126.18.4165 [doi]
PST - ppublish
SO  - Development. 1999 Sep;126(18):4165-73. doi: 10.1242/dev.126.18.4165.