PMID- 10456937
OWN - NLM
STAT- MEDLINE
DCOM- 19991005
LR  - 20181113
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 67
IP  - 9
DP  - 1999 Sep
TI  - Molecular cloning and characterization of rat genes encoding homologues of human 
      beta-defensins.
PG  - 4827-33
AB  - beta-Defensins are cationic peptides with broad-spectrum antimicrobial activity
      that may play a role in mucosal defenses of several organs. They have been
      isolated in several species, and in humans, two beta-defensins have been
      identified. Here, we report the identification of two genes encoding
      beta-defensin homologues in the rat. Partial cDNAs were found by searching the
      expressed-sequence-tag database, and primers were designed to generate
      full-length mRNA coding sequences. One gene was highly similar to the human
      beta-defensin-1 (HBD-1) gene and mouse beta-defensin-1 gene at both the nucleic
      acid and amino acid levels and was termed rat beta-defensin-1 (RBD-1). The other 
      gene, named RBD-2, was homologous to the HBD-2 and bovine tracheal antimicrobial 
      peptide (TAP) genes. The predicted prepropeptides were strongly cationic, were 69
      and 63 residues in length for RBD-1 and RBD-2, respectively, and contained the
      six-cysteine motif characteristic of beta-defensins. The beta-defensin genes
      mapped closely on rat chromosome 16 and were closely linked to the
      alpha-defensins genes, suggesting that they are part of a gene cluster, similar
      to the organization reported for humans. Northern blot analysis showed that both 
      RBD-1 and RBD-2 mRNA transcripts were approximately 0.5 kb in length; RBD-1 mRNA 
      was abundantly transcribed in the rat kidney, while RBD-2 was prevalent in the
      lung. Reverse transcription-PCR indicated that RBD-1 and RBD-2 mRNAs were
      distributed in a variety of other tissues. In the lung, RBD-1 mRNA expression
      localized to the tracheal epithelium while RBD-2 was expressed in alveolar type
      II cells. In conclusion, we characterized two novel beta-defensin homologues in
      the rat. The rat may be a useful model to investigate the function and
      contribution of beta-defensins to host defense in the lung, kidney, and other
      tissues.
FAU - Jia, H P
AU  - Jia HP
AD  - Department of Pediatrics, University of Iowa College of Medicine, Iowa City,
      Iowa, USA.
FAU - Mills, J N
AU  - Mills JN
FAU - Barahmand-Pour, F
AU  - Barahmand-Pour F
FAU - Nishimura, D
AU  - Nishimura D
FAU - Mallampali, R K
AU  - Mallampali RK
FAU - Wang, G
AU  - Wang G
FAU - Wiles, K
AU  - Wiles K
FAU - Tack, B F
AU  - Tack BF
FAU - Bevins, C L
AU  - Bevins CL
FAU - McCray, P B Jr
AU  - McCray PB Jr
LA  - eng
SI  - GENBANK/AF068860
SI  - GENBANK/AF068861
GR  - P50 HL-61234-01/HL/NHLBI NIH HHS/United States
GR  - R01 AI032738/AI/NIAID NIH HHS/United States
GR  - HL61234/HL/NHLBI NIH HHS/United States
GR  - AI-32234/AI/NIAID NIH HHS/United States
GR  - P50 HL061234/HL/NHLBI NIH HHS/United States
GR  - R37 AI032738/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (DEFB1 protein, human)
RN  - 0 (DNA, Complementary)
RN  - 0 (Defensins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (beta-Defensins)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Bronchoalveolar Lavage Fluid
MH  - Cattle
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Defensins
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization
MH  - Mice
MH  - Molecular Sequence Data
MH  - Proteins/*genetics/metabolism
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Homology, Amino Acid
MH  - Sodium Chloride
MH  - Tissue Distribution
MH  - *beta-Defensins
PMC - PMC96815
EDAT- 1999/08/24 10:00
MHDA- 2001/03/28 10:01
CRDT- 1999/08/24 10:00
PHST- 1999/08/24 10:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/08/24 10:00 [entrez]
PST - ppublish
SO  - Infect Immun. 1999 Sep;67(9):4827-33.