PMID- 10456837
OWN - NLM
STAT- MEDLINE
DCOM- 19990920
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 61
IP  - 3
DP  - 1999 Sep
TI  - Temporal and tissue-specific expression of kallikrein (Klk) genes and
      identification of a novel Klk messenger ribonucleic acid transcript during early 
      development in the mouse.
PG  - 621-8
AB  - The kallikreins are a multigene family of serine proteases that act on a diverse 
      number of substrates, including several growth factors and extracellular matrix
      (ECM) glycoproteins and proteinases. Recently, this family has been implicated in
      the process of early development and embryo implantation. In this study, we used 
      reverse transcription-polymerase chain reaction with gene-specific primers and
      Southern hybridization to elucidate the temporal and tissue-specific expression
      patterns of the mouse kallikreins Klk1, Klk3, Klk5, Klk9, and Klk21 during early 
      development in the embryo, uterus, and decidua. We observed the expression of
      Klk1 (tissue kallikrein), Klk3 (gamma-nerve growth factor), Klk9 (epidermal
      growth factor-binding protein), and Klk21 in the early conceptus (until the
      2-cell stage). Only Klk21 continued to be expressed in the blastocyst until Day
      7.5 of pregnancy. Expression of Klk9 reappeared at Day 7.5 and was consistently
      detected until Day 11, the last day studied; Klk1 was again expressed in the
      embryo from Day 9.5, with decreased levels by Day 11. In contrast, in the uterus 
      or decidua, there was no expression of Klk1 until Day 7.5, when mRNA transcripts 
      were abundant; transcripts then decreased in the Day 9.5 and Day 11 uterus.
      Expression of Klk21 in the uterus and decidua displayed a similar pattern but was
      detected at much lower levels. Interestingly, a novel Klk21-like mRNA was also
      detected in uterine tissue samples but not in embryonic samples; Klk3, Klk5, and 
      Klk9 were not consistently expressed in the uterus or decidua over this time.
      This is the first report of the expression of specific kallikreins during early
      development. The distinct gene- and tissue-specific expression patterns presented
      in this study, in conjunction with the well-characterized roles of kallikreins in
      regulation of protein activation, ECM degradation, and proliferative events,
      suggests the involvement of the kallikrein gene family during early development.
FAU - Chan, C S
AU  - Chan CS
AD  - Centre for Molecular Biotechnology, School of Life Sciences, Queensland
      University of Technology, Brisbane, Queensland 4001, Australia.
FAU - Harvey, M B
AU  - Harvey MB
FAU - Clements, J A
AU  - Clements JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.21.- (Kallikreins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blastocyst/metabolism
MH  - Blotting, Southern
MH  - Decidua/metabolism
MH  - *Embryonic and Fetal Development
MH  - Female
MH  - *Gene Expression
MH  - Kallikreins/chemistry/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Pregnancy
MH  - RNA, Messenger/*analysis/chemistry
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Uterus/metabolism
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1095/biolreprod61.3.621 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Sep;61(3):621-8. doi: 10.1095/biolreprod61.3.621.