PMID- 10456793
OWN - NLM
STAT- MEDLINE
DCOM- 19990922
LR  - 20220801
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 18
IP  - 3
DP  - 1999
TI  - Identification of a gene cluster within the genome of Chilo iridescent virus
      encoding enzymes involved in viral DNA replication and processing.
PG  - 243-64
AB  - The nucleotide sequence of the genome of Chilo iridescent virus (CIV) between the
      genome coordinates 0.974 and 0.101 comprising 27,079 bp was determined.
      Computer-assisted analysis of the DNA sequence of this particular region of the
      CIV genome revealed the presence of 42 potential open reading frames (ORFs) with 
      coding capacities for polypeptides ranging from 50 to 1,273 amino acid residues. 
      The analysis of the amino acid sequences deduced from the individual ORFs
      resulted in the identification of 10 potential viral genes that show significant 
      homology to functionally characterized proteins of other species. A cluster of
      five viral genes that encode enzymes involved in the viral DNA replication was
      identified including the DNA topoisomerase II (A039L,1,132 amino acids (aa)), the
      DNA polymerase (ORF A031L,1,273 aa), a helicase (ORF A027L, 530 aa), a nucleoside
      triphosphatase I (ORF A025L, 1,171 aa), and an exonuclease II (ORF A019L, 624aa),
      all ORFs possessing the same genomic orientation. The DNA polymerase of CIV
      showed the highest homology (24.8% identity) to the DNA polymerase of
      lymphocystis disease virus lymphocystis disease virus 1 (LCDV-1), a member of the
      family Iridoviridae, indicating the close relatedness of the two viruses. In
      addition, four putative gene products were found to be significantly homologous
      to previously identified hypothetical proteins of CIV.
FAU - Muller, K
AU  - Muller K
AD  - Institut fur Medizinische Virologie, Universitat Heidelberg, Federal Republic of 
      Germany.
FAU - Tidona, C A
AU  - Tidona CA
FAU - Darai, G
AU  - Darai G
LA  - eng
SI  - GENBANK/AF083915
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (DNA Primers)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.11.- (exodeoxyribonuclease II)
RN  - EC 3.6.1.- (PIF1 protein, S cerevisiae)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 5.99.1.3 (DNA Topoisomerases, Type II)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - DNA Helicases/genetics
MH  - DNA Primers
MH  - DNA Replication/*genetics
MH  - DNA Topoisomerases, Type II/genetics
MH  - DNA-Directed DNA Polymerase/genetics
MH  - Exodeoxyribonucleases/genetics
MH  - Genome, Viral
MH  - Iridoviridae/enzymology/*genetics
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Open Reading Frames
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1023/a:1008072319875 [doi]
PST - ppublish
SO  - Virus Genes. 1999;18(3):243-64. doi: 10.1023/a:1008072319875.