PMID- 10456331
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 456
IP  - 2
DP  - 1999 Aug 6
TI  - Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the
      protein in mouse testis.
PG  - 315-21
AB  - Tektins are a class of proteins that form filamentous polymers in the walls of
      ciliary and flagellar microtubules. We report here the molecular cloning of a new
      member of the tektin family, tektin-t, identified from a mouse haploid germ
      cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino 
      acids possessing RSNVELCRD, the conserved sequence of tektin family proteins.
      Western blotting showed a single band having a molecular weight of 86 kDa in the 
      mouse testis. Immunohistochemistry of the testis showed that tektin-t is
      localized in the flagella of elongating spermatids from developmental step 15 to 
      maturity.
FAU - Iguchi, N
AU  - Iguchi N
AD  - Department of Science for Laboratory Animal Experimentation, Research Institute
      for Microbial Diseases, Osaka University, Suita City, Japan.
FAU - Tanaka, H
AU  - Tanaka H
FAU - Fujii, T
AU  - Fujii T
FAU - Tamura, K
AU  - Tamura K
FAU - Kaneko, Y
AU  - Kaneko Y
FAU - Nojima, H
AU  - Nojima H
FAU - Nishimune, Y
AU  - Nishimune Y
LA  - eng
SI  - GENBANK/AB027138
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Microtubule Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (tektins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/*genetics
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Microtubule Proteins/analysis/*genetics/*metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Sperm Tail/metabolism
MH  - Spermatozoa/*metabolism
MH  - Testis/*metabolism
MH  - Tissue Distribution
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - S0014-5793(99)00967-9 [pii]
AID - 10.1016/s0014-5793(99)00967-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Aug 6;456(2):315-21. doi: 10.1016/s0014-5793(99)00967-9.