PMID- 10456321
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 456
IP  - 2
DP  - 1999 Aug 6
TI  - Synthesis of plasmalogens in eye lens epithelial cells.
PG  - 263-8
AB  - The present paper describes cloning and sequencing of the mouse cDNA encoding
      dihydroxyacetonephosphate acyltransferase (DAPAT), the peroxisomal key enzyme of 
      plasmalogen (PM) biosynthesis. Using monospecific antibodies, we localized DAPAT 
      and alkyl dihydroxyacetonephosphate synthase to peroxisomes of mouse lens
      epithelial cells (LECs) and determined their enzymatic activity. By electrospray 
      ionization mass spectrometry of mouse lens lipid extracts, we identified
      phosphatidyl ethanolamine including plasmenyl ethanolamine species as major
      constituents. Our data demonstrate the capacity of LECs to synthesize PMs and the
      high coincidence between deficiency of PM and early manifestation of cataract in 
      patients with peroxisomal disorders suggests that ether-bonded lipids may play an
      important role in maintaining lens transparency.
FAU - Thai, T P
AU  - Thai TP
AD  - Biochemiezentrum Heidelberg, Universitat Heidelberg, Germany.
FAU - Rodemer, C
AU  - Rodemer C
FAU - Worsch, J
AU  - Worsch J
FAU - Hunziker, A
AU  - Hunziker A
FAU - Gorgas, K
AU  - Gorgas K
FAU - Just, W W
AU  - Just WW
LA  - eng
SI  - GENBANK/AJ002190
SI  - GENBANK/AJ132012
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Plasmalogens)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.42 (glycerone-phosphate O-acyltransferase)
RN  - EC 2.5.- (Alkyl and Aryl Transferases)
RN  - EC 2.5.1.26 (alkylglycerone-phosphate synthase)
SB  - IM
MH  - Acyltransferases/genetics/metabolism
MH  - Alkyl and Aryl Transferases/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cataract/etiology/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Epithelial Cells/metabolism/ultrastructure
MH  - Humans
MH  - Lens, Crystalline/cytology/*metabolism
MH  - Mice
MH  - Microbodies/enzymology
MH  - Microscopy, Electron
MH  - Molecular Sequence Data
MH  - Plasmalogens/*biosynthesis
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - S0014-5793(99)00968-0 [pii]
AID - 10.1016/s0014-5793(99)00968-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Aug 6;456(2):263-8. doi: 10.1016/s0014-5793(99)00968-0.