PMID- 10455179
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20191210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 35
DP  - 1999 Aug 27
TI  - Molecular characterization of a novel basement membrane-associated proteoglycan, 
      leprecan.
PG  - 25004-17
AB  - A monoclonal antibody was used in early studies to identify a novel chondroitin
      sulfate proteoglycan, secreted by L-2 cells, the core protein of which was
      approximately 100 kDa. To characterize this proteoglycan core protein at the
      molecular level, an L-2 cell cDNA library was probed by expression screening and 
      solution hybridization. Northern blot analysis assigned transcript size to
      approximately 3.1 kilobases and, after contig assembly, the coding region of the 
      mRNA corresponded to 2.18 kilobases. Immunoassays were performed to confirm the
      identity of this sequence, using a polyclonal antibody raised against an
      expressed fusion protein encoded by sequence representing the carboxyl half of
      the molecule. The antibody recognized the core protein in Western blots after
      prior digestion of the intact proteoglycan with chondroitinase ABC.
      Immunostaining tissue sections with the same antibody localized the proteoglycan 
      to basement membranes, and expression of the entire sequence in Chinese hamster
      ovary K-1 cells showed that the protein encoded by the sequence secreted as a
      chondroitin sulfate proteoglycan. The core protein not only has motifs permitting
      glycosylation as a proteoglycan, but also possesses the endoplasmic reticulum
      retrieval signal, KDEL, which suggests that, in addition to its role as a
      basement membrane component, it may also participate in the secretory pathway of 
      cells.
FAU - Wassenhove-McCarthy, D J
AU  - Wassenhove-McCarthy DJ
AD  - Department of Pathology, School of Medicine, Louisiana State University Medical
      Center, Shreveport, Louisiana 71130, USA.
FAU - McCarthy, K J
AU  - McCarthy KJ
LA  - eng
SI  - GENBANK/AF087433
GR  - 1-RO1-DK48055/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 0 (DNA, Complementary)
RN  - 0 (Lepre1 protein, rat)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proteoglycans)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 63231-63-0 (RNA)
RN  - EC 1.14.11.- (Prolyl Hydroxylases)
RN  - EC 1.14.11.7 (P3H1 protein, human)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Basement Membrane/*chemistry
MH  - CHO Cells
MH  - Chondroitin ABC Lyase/metabolism
MH  - Chondroitin Sulfate Proteoglycans/*chemistry/genetics
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Dermatan Sulfate/chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney Glomerulus/cytology
MH  - *Membrane Glycoproteins
MH  - Molecular Sequence Data
MH  - Prolyl Hydroxylases
MH  - Protein Structure, Secondary
MH  - Proteoglycans/*chemistry/genetics
MH  - RNA/analysis
MH  - Rats
MH  - Sequence Alignment
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1074/jbc.274.35.25004 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 27;274(35):25004-17. doi: 10.1074/jbc.274.35.25004.