PMID- 10455115
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 35
DP  - 1999 Aug 27
TI  - Redox regulation of cell signaling by selenocysteine in mammalian thioredoxin
      reductases.
PG  - 24522-30
AB  - The intracellular generation of reactive oxygen species, together with the
      thioredoxin and glutathione systems, is thought to participate in redox signaling
      in mammalian cells. The activity of thioredoxin is dependent on the redox status 
      of thioredoxin reductase (TR), the activity of which in turn is dependent on a
      selenocysteine residue. Two mammalian TR isozymes (TR2 and TR3), in addition to
      that previously characterized (TR1), have now been identified in humans and mice.
      All three TR isozymes contain a selenocysteine residue that is located in the
      penultimate position at the carboxyl terminus and which is encoded by a UGA
      codon. The generation of reactive oxygen species in a human carcinoma cell line
      was shown to result in both the oxidation of the selenocysteine in TR1 and a
      subsequent increase in the expression of this enzyme. These observations identify
      the carboxyl-terminal selenocysteine of TR1 as a cellular redox sensor and
      support an essential role for mammalian TR isozymes in redox-regulated cell
      signaling.
FAU - Sun, Q A
AU  - Sun QA
AD  - Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA.
FAU - Wu, Y
AU  - Wu Y
FAU - Zappacosta, F
AU  - Zappacosta F
FAU - Jeang, K T
AU  - Jeang KT
FAU - Lee, B J
AU  - Lee BJ
FAU - Hatfield, D L
AU  - Hatfield DL
FAU - Gladyshev, V N
AU  - Gladyshev VN
LA  - eng
SI  - GENBANK/AF171053
SI  - GENBANK/AF171054
SI  - GENBANK/AF171055
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Fluoresceins)
RN  - 0 (Isoenzymes)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Recombinant Proteins)
RN  - 0CH9049VIS (Selenocysteine)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 63368-54-7 (5-iodoacetamidofluorescein)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.- (TXNRD3 protein, human)
RN  - EC 1.8.1.9 (Thioredoxin-Disulfide Reductase)
RN  - GE3IBT7BMN (Dinitrochlorobenzene)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Dinitrochlorobenzene/chemistry
MH  - Epidermal Growth Factor/pharmacology
MH  - Fluoresceins/chemistry
MH  - Gene Expression Regulation, Enzymologic/genetics
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Isoenzymes/chemistry/genetics
MH  - Liver/enzymology
MH  - Male
MH  - Mass Spectrometry
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Peptide Fragments/analysis
MH  - Peroxidases
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Recombinant Proteins/genetics
MH  - Selenocysteine/*metabolism
MH  - *Signal Transduction
MH  - Testis/enzymology
MH  - Thioredoxin-Disulfide Reductase/chemistry/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1074/jbc.274.35.24522 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 27;274(35):24522-30. doi: 10.1074/jbc.274.35.24522.