PMID- 10455105
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 35
DP  - 1999 Aug 27
TI  - Association of neuronal calcium channels with modular adaptor proteins.
PG  - 24453-6
AB  - Presynaptic voltage-gated calcium (Ca(2+)) channels mediate Ca(2+) influx into
      the presynaptic terminal that triggers synaptic vesicle fusion and
      neurotransmitter release. The immediate proximity of Ca(2+) channels to the
      synaptic vesicle release apparatus is critical for rapid and efficient synaptic
      transmission. In a series of biochemical experiments, we demonstrate a specific
      association of the cytosolic carboxyl terminus of the N-type Ca(2+) channel
      pore-forming alpha(1B) subunit with the modular adaptor proteins Mint1 and CASK. 
      The carboxyl termini of alpha(1B) bind to the first PDZ domain of Mint1
      (Mint1-1). The proline-rich region present in the carboxyl termini of alpha(1B)
      binds to the SH3 domain of CASK. Mint1-1 is specific for the E/D-X-W-C/S-COOH
      consensus, which defines a novel class of PDZ domains (class III). The Mint1-1
      PDZ domain-binding motif is present only in the "long" carboxyl-terminal splice
      variants of N-type (alpha(1B)) and P/Q-type (alpha(1A)) Ca(2+) channels, but not 
      in R-type (alpha(1E)) or L-type (alpha(1C)) Ca(2+) channels. Our results directly
      link presynaptic Ca(2+) channels to a macromolecular complex formed by modular
      adaptor proteins at synaptic junction and advance our understanding of coupling
      between cell adhesion and synaptic vesicle exocytosis.
FAU - Maximov, A
AU  - Maximov A
AD  - Department of Physiology, University of Texas Southwestern Medical Center,
      Dallas, Texas 75235, USA.
FAU - Sudhof, T C
AU  - Sudhof TC
FAU - Bezprozvanny, I
AU  - Bezprozvanny I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Apba1 protein, rat)
RN  - 0 (Calcium Channels)
RN  - 0 (Carrier Proteins)
RN  - 0 (Ligands)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 2.7.11.1 (CASK kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.4.4 (Nucleoside-Phosphate Kinase)
RN  - EC 2.7.4.8 (Guanylate Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/metabolism
MH  - COS Cells
MH  - Calcium/metabolism
MH  - Calcium Channels/*metabolism
MH  - *Calcium-Calmodulin-Dependent Protein Kinases
MH  - Carrier Proteins/*metabolism
MH  - Cell Adhesion
MH  - Exocytosis
MH  - Guanylate Kinases
MH  - Ligands
MH  - Membrane Proteins
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*metabolism
MH  - Nucleoside-Phosphate Kinase/*metabolism
MH  - Protein Binding
MH  - Rats
MH  - Signal Transduction
MH  - Synaptic Transmission
MH  - Yeasts
MH  - src Homology Domains
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1074/jbc.274.35.24453 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 27;274(35):24453-6. doi: 10.1074/jbc.274.35.24453.