PMID- 10455013
OWN - NLM
STAT- MEDLINE
DCOM- 19991101
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 342 ( Pt 2)
DP  - 1999 Sep 1
TI  - Phosphorylation of Ser-241 is essential for the activity of
      3-phosphoinositide-dependent protein kinase-1: identification of five sites of
      phosphorylation in vivo.
PG  - 287-92
AB  - 3-phosphoinositide-dependent protein kinase-1 (PDK1) expressed in unstimulated
      293 cells was phosphorylated at Ser-25, Ser-241, Ser-393, Ser-396 and Ser-410 and
      the level of phosphorylation of each site was unaffected by stimulation with
      insulin-like growth factor-1. Mutation of Ser-241 to Ala abolished PDK1 activity,
      whereas mutation of the other phosphorylation sites individually to Ala did not
      affect PDK1 activity. Ser-241, unlike the other phosphorylation sites on PDK1,
      was resistant to dephosphorylation by protein phosphatase 2A(1). Ser-241 lies in 
      the activation loop of the PDK1 kinase domain between subdomains VII and VIII in 
      the equivalent position to the site that PDK1 phosphorylates on its protein
      kinase substrates. PDK1 expressed in bacteria was active and phosphorylated at
      Ser-241, suggesting that PDK1 can phosphorylate itself at this site, leading to
      its own activation.
FAU - Casamayor, A
AU  - Casamayor A
AD  - MRC Protein Phosphorylation Unit, Department of Biochemistry, University of
      Dundee, Dundee DD1 5EH, Scotland, U.K.
FAU - Morrice, N A
AU  - Morrice NA
FAU - Alessi, D R
AU  - Alessi DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Recombinant Fusion Proteins)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.1 (3-Phosphoinositide-Dependent Protein Kinases)
RN  - EC 2.7.11.1 (PDPK1 protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - 3-Phosphoinositide-Dependent Protein Kinases
MH  - Amino Acid Sequence
MH  - Binding Sites/genetics
MH  - Cell Line
MH  - Enzyme Activation
MH  - Escherichia coli/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Peptide Mapping
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/*chemistry/genetics/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Serine/chemistry
MH  - Transfection
PMC - PMC1220463
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Sep 1;342 ( Pt 2):287-92.