PMID- 10454540
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 9
DP  - 1999 Sep
TI  - Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae 
      reveal a specific role in meiosis.
PG  - 5930-42
AB  - Xrn1p of Saccharomyces cerevisiae is a major cytoplasmic RNA turnover exonuclease 
      which is evolutionarily conserved from yeasts to mammals. Deletion of the XRN1 
      gene causes pleiotropic phenotypes, which have been interpreted as indirect 
      consequences of the RNA turnover defect. By sequence comparisons, we have 
      identified three loosely defined, common 5'-3' exonuclease motifs. The 
      significance of motif II has been confirmed by mutant analysis with Xrn1p. The 
      amino acid changes D206A and D208A abolish singly or in combination the 
      exonuclease activity in vivo. These mutations show separation of function. They 
      cause identical phenotypes to that of xrn1Delta in vegetative cells but do not 
      exhibit the severe meiotic arrest and the spore lethality phenotype typical for 
      the deletion. In addition, xrn1-D208A does not cause the severe reduction in 
      meiotic popout recombination in a double mutant with dmc1 as does xrn1Delta. 
      Biochemical analysis of the DNA binding, exonuclease, and homologous pairing 
      activity of purified mutant enzyme demonstrated the specific loss of exonuclease 
      activity. However, the mutant enzyme is competent to promote in vitro assembly of 
      tubulin into microtubules. These results define a separable and specific function 
      of Xrn1p in meiosis which appears unrelated to its RNA turnover function in 
      vegetative cells.
FAU - Solinger, J A
AU  - Solinger JA
AD  - Institute of General Microbiology, University of Bern, CH-3012 Bern, Switzerland.
FAU - Pascolini, D
AU  - Pascolini D
FAU - Heyer, W D
AU  - Heyer WD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Fungal)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.1.- (Exoribonucleases)
RN  - EC 3.1.11.- (XRN1 protein, S cerevisiae)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Catalytic Domain/genetics
MH  - Conserved Sequence
MH  - DNA Primers/genetics
MH  - DNA, Fungal/chemistry/genetics/metabolism
MH  - Exoribonucleases/*genetics
MH  - Meiosis/*genetics/*physiology
MH  - Microtubules/metabolism
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Phenotype
MH  - RNA, Fungal/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Saccharomyces cerevisiae/cytology/*enzymology/*genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
PMC - PMC84450
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 0525 [pii]
AID - 10.1128/MCB.19.9.5930 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Sep;19(9):5930-42. doi: 10.1128/MCB.19.9.5930.