PMID- 10454528
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20171116
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 290
IP  - 3
DP  - 1999 Sep
TI  - Functional characteristics and tissue distribution pattern of organic cation 
      transporter 2 (OCTN2), an organic cation/carnitine transporter.
PG  - 1482-92
AB  - We have demonstrated in the present study that novel organic cation transporter 
      (OCTN) 2 is a transporter for organic cations as well as carnitine. OCTN2 
      transports organic cations without involving Na(+), but it transports carnitine 
      only in the presence of Na(+). The ability to transport organic cations and 
      carnitine is demonstrable with human, rat, and mouse OCTN2s. Na(+) does not 
      influence the affinity of OCTN2 for organic cations, but it increases the 
      affinity severalfold for carnitine. The short-chain acyl esters of carnitine are 
      also transported by OCTN2. Two mutations, M352R and P478L, in human OCTN2 are 
      associated with loss of transport function, but the protein expression of these 
      mutants is comparable to that of the wild-type human OCTN2. In situ hybridization 
      in the rat shows that OCTN2 is expressed in the proximal and distal tubules and 
      in the glomeruli in the kidney, in the myocardium, valves, and arterioles in the 
      heart, in the labyrinthine layer of the placenta, and in the cortex, hippocampus, 
      and cerebellum in the brain. This is the first report that OCTN2 is a 
      Na(+)-independent organic cation transporter as well as a Na(+)-dependent 
      carnitine transporter and that OCTN2 is expressed not only in the heart, kidney, 
      and placenta but also in the brain.
FAU - Wu, X
AU  - Wu X
AD  - Department of Biochemistry and Molecular Biology, Medical College of Georgia, 
      Augusta, Georgia, USA.
FAU - Huang, W
AU  - Huang W
FAU - Prasad, P D
AU  - Prasad PD
FAU - Seth, P
AU  - Seth P
FAU - Rajan, D P
AU  - Rajan DP
FAU - Leibach, F H
AU  - Leibach FH
FAU - Chen, J
AU  - Chen J
FAU - Conway, S J
AU  - Conway SJ
FAU - Ganapathy, V
AU  - Ganapathy V
LA  - eng
SI  - GENBANK/AF110416
SI  - GENBANK/AF110417
GR  - DA 10045/DA/NIDA NIH HHS/United States
GR  - HD 33347/HD/NICHD NIH HHS/United States
GR  - HL 60104/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Organic Cation Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SLC22A5 protein, human)
RN  - 0 (Slc22a5 protein, mouse)
RN  - 0 (Slc22a5 protein, rat)
RN  - 0 (Solute Carrier Family 22 Member 5)
RN  - 17298-37-2 (propionylcarnitine)
RN  - 66-40-0 (Tetraethylammonium)
RN  - 6DH1W9VH8Q (Acetylcarnitine)
RN  - S7UI8SM58A (Carnitine)
SB  - IM
MH  - Acetylcarnitine/pharmacokinetics
MH  - Animals
MH  - Biological Transport, Active
MH  - Carnitine/analogs & derivatives/pharmacokinetics
MH  - Carrier Proteins/biosynthesis/metabolism/*physiology
MH  - Humans
MH  - In Situ Hybridization
MH  - Kidney/metabolism
MH  - Membrane Proteins/biosynthesis/metabolism/*physiology
MH  - Mice
MH  - Myocardium/metabolism
MH  - Organ Specificity
MH  - *Organic Cation Transport Proteins
MH  - Placenta/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Solute Carrier Family 22 Member 5
MH  - Tetraethylammonium/pharmacokinetics
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
PST - ppublish
SO  - J Pharmacol Exp Ther. 1999 Sep;290(3):1482-92.