PMID- 10454141
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 270
IP  - 1
DP  - 1999 Jul 23
TI  - Expression of wild-type and V210I mutant prion protein in human neuroblastoma 
      cells.
PG  - 41-4
AB  - The conversion of the host-encoded prion protein (PrPc) into the insoluble, 
      protease-resistant isoform (PrPsc) is the main pathogenic mechanism of 
      transmissible spongiform encephalopathies. They are fatal neurodegenerative 
      disorders, which in human occur as sporadic, inherited or familial forms. These 
      last forms are linked to insert or point mutations of PrPc which may facilitate 
      the spontaneous conversion into PrPsc. We have established stably transfected 
      human neuroblastoma cells (SH-SY5Y) expressing mutant V210I, or wild-type PrPc. 
      Both proteins were expressed and attached to the cell surface. The mutation in 
      position 210 did not alter the biochemical properties of the protein in 
      comparison with the wild-type protein nor induced any conformational changes 
      similar to those observed in PrPsc.
FAU - Vetrugno, V
AU  - Vetrugno V
AD  - Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy.
FAU - Malchow, M
AU  - Malchow M
FAU - Liu, Q
AU  - Liu Q
FAU - Marziali, G
AU  - Marziali G
FAU - Battistini, A
AU  - Battistini A
FAU - Pocchiari, M
AU  - Pocchiari M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Amyloid)
RN  - 0 (PRNP protein, human)
RN  - 0 (PrPC Proteins)
RN  - 0 (PrPSc Proteins)
RN  - 0 (Prion Proteins)
RN  - 0 (Prions)
RN  - 0 (Protein Precursors)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
SB  - IM
MH  - Amino Acid Substitution
MH  - Amyloid/biosynthesis/genetics
MH  - Brain Neoplasms
MH  - Cell Membrane/metabolism
MH  - Humans
MH  - Neuroblastoma
MH  - Open Reading Frames
MH  - Phosphatidylinositol Diacylglycerol-Lyase
MH  - Point Mutation
MH  - PrPC Proteins/biosynthesis/*genetics
MH  - PrPSc Proteins/biosynthesis/*genetics
MH  - Prion Proteins
MH  - Prions
MH  - Protein Precursors/biosynthesis/genetics
MH  - Recombinant Proteins/biosynthesis
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Type C Phospholipases/metabolism
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - S0304394099004607 [pii]
AID - 10.1016/s0304-3940(99)00460-7 [doi]
PST - ppublish
SO  - Neurosci Lett. 1999 Jul 23;270(1):41-4. doi: 10.1016/s0304-3940(99)00460-7.