PMID- 10452946
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20211203
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 236
IP  - 2
DP  - 1999 Aug 20
TI  - Cloning and characterization of the alternative promoter regions of the human
      LIMK2 gene responsible for alternative transcripts with tissue-specific
      expression.
PG  - 259-71
AB  - We previously isolated the human LIMK2 gene and identified two alternative
      transcripts, LIMK2a and LIMK2b, which were differentially regulated in a
      tissue-specific manner. To examine this differential tissue-specific expression
      in detail, an RNase protection assay was performed, which demonstrated three
      expression patterns of the LIMK2 isoforms. In digestive organs, the LIMK2a
      transcripts were preferentially expressed in fetal and adult tissues; in brain
      and lung, the LIMK2a transcript was predominantly expressed only in fetal tissue,
      and in placenta, the LIMK2b transcript was expressed more abundantly than that of
      LIMK2a. To further investigate this mechanism and the transcription factors
      involved, we isolated the two distinct 5' upstream regions from the phage genomic
      library and found that both LIMK2a and 2b promoters have a single major
      transcription initiation site and the characteristics of a TATA-less promoter. A 
      luciferase reporter assay of the transcriptional activity revealed positive as
      well as negative regulatory regions within both promoters. The co-transfection
      assay suggested that the MZF-1 might regulate the expression of the LIMK2
      isoforms in a different manner. The RORalpha1 might also be involved in the
      transcriptional regulation of the LIMK2b isoform. The genomic structure of the
      LIMK2 gene was also determined. These findings should lead to a better
      understanding of the possibly diverse functions of the LIMK family.
FAU - Nomoto, S
AU  - Nomoto S
AD  - Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute,
      1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan.
FAU - Tatematsu, Y
AU  - Tatematsu Y
FAU - Takahashi, T
AU  - Takahashi T
FAU - Osada, H
AU  - Osada H
LA  - eng
SI  - GENBANK/AB016655
SI  - GENBANK/AB016656
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MZF1 protein, human)
RN  - 0 (Nuclear Receptor Subfamily 1, Group F, Member 1)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.1 (LIMK2 protein, human)
RN  - EC 2.7.11.1 (Lim Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - *Alternative Splicing
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*genetics
MH  - Gene Expression Regulation
MH  - Gene Library
MH  - Genes, Reporter
MH  - Humans
MH  - Kruppel-Like Transcription Factors
MH  - Lim Kinases
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Nuclear Receptor Subfamily 1, Group F, Member 1
MH  - Organ Specificity
MH  - Placenta/metabolism
MH  - *Promoter Regions, Genetic
MH  - Protein Isoforms
MH  - Protein Serine-Threonine Kinases
MH  - Receptors, Cytoplasmic and Nuclear/genetics
MH  - Regulatory Sequences, Nucleic Acid
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Distribution
MH  - Trans-Activators/genetics
MH  - Transcription Factors/genetics
MH  - Transcription, Genetic
MH  - Transcriptional Activation
MH  - Tumor Cells, Cultured
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - S0378111999002802 [pii]
AID - 10.1016/s0378-1119(99)00280-2 [doi]
PST - ppublish
SO  - Gene. 1999 Aug 20;236(2):259-71. doi: 10.1016/s0378-1119(99)00280-2.