PMID- 10452889
OWN - NLM
STAT- MEDLINE
DCOM- 19991004
LR  - 20090929
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 291
IP  - 4
DP  - 1999 Aug 27
TI  - Gene organisation, sequence variation and isochore structure at the centromeric
      boundary of the human MHC.
PG  - 789-99
AB  - We have mapped and sequenced the region immediately centromeric of the human
      major histocompatibility complex (MHC). A cluster of 13 genes/pseudogenes was
      identified in a 175 kb PAC linking the TAPASIN locus with the class II region. It
      includes two novel human genes (BING4 and SACM2L) and a thus far unnoticed human 
      leucocyte antigen (HLA) class II pseudogene, termed HLA-DPA3. Analysis of the G+C
      content revealed an isochore boundary which, together with the previously
      reported telomeric boundary, defines the MHC class II region as one of the first 
      completely sequenced isochores in the human genome. Comparison of the sequence
      with limited sequence from other cell lines shows that the high sequence
      variation found within the classical class II region extends beyond the
      identified isochore boundary leading us to propose the concept of an "extended
      MHC". By comparative analysis, we have precisely identified the mouse/human
      synteny breakpoint at the centromeric end of the extended MHC class II region
      between the genes HSET and PHF1.
CI  - Copyright 1999 Academic Press.
FAU - Stephens, R
AU  - Stephens R
AD  - Immunology, Department of Pathology, University of Cambridge, Tennis Court Road, 
      Cambridge, CB2 1QP, UK.
FAU - Horton, R
AU  - Horton R
FAU - Humphray, S
AU  - Humphray S
FAU - Rowen, L
AU  - Rowen L
FAU - Trowsdale, J
AU  - Trowsdale J
FAU - Beck, S
AU  - Beck S
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (DNA, Complementary)
RN  - 0 (HLA-DP Antigens)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Composition
MH  - Base Sequence
MH  - Centromere/genetics
MH  - Chromosome Mapping
MH  - DNA, Complementary/chemistry/genetics
MH  - Genes, MHC Class II
MH  - Genetic Variation
MH  - Genome, Human
MH  - HLA-DP Antigens/genetics
MH  - Humans
MH  - *Major Histocompatibility Complex
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Pseudogenes
MH  - Sequence Homology, Amino Acid
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1006/jmbi.1999.3004 [doi]
AID - S0022-2836(99)93004-8 [pii]
PST - ppublish
SO  - J Mol Biol. 1999 Aug 27;291(4):789-99. doi: 10.1006/jmbi.1999.3004.