PMID- 10452533
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 456
IP  - 1
DP  - 1999 Jul 30
TI  - The low Mr phosphotyrosine protein phosphatase behaves differently when
      phosphorylated at Tyr131 or Tyr132 by Src kinase.
PG  - 73-8
AB  - The low molecular weight phosphotyrosine protein phosphatase (LMW-PTP) is
      phosphorylated by Src and Src-related kinases both in vitro and in vivo; in
      Jurkat cells, and in NIH-3T3 cells, it becomes tyrosine-phosphorylated upon
      stimulation by PDGF. In this study we show that pp60Src phosphorylates in vitro
      the enzyme at two tyrosine residues, Tyr131 and Tyr132, previously indicated as
      the main phosphorylation sites of the enzyme, whereas phosphorylation by the
      PDGF-R kinase is much less effective and not specific. The effects of LMW-PTP
      phosphorylation at each tyrosine residue were investigated by using Tyr131 and
      Tyr132 mutants. We found that the phosphorylation at either residue has differing
      effects on the enzyme behaviour: Tyr131 phosphorylation is followed by a strong
      (about 25-fold) increase of the enzyme specific activity, whereas phosphorylation
      at Tyr132 leads to Grb2 recruitment. These differing effects are discussed on the
      light of the enzyme structure.
FAU - Bucciantini, M
AU  - Bucciantini M
AD  - Department of Biochemical Sciences, University of Florence, Italy.
FAU - Chiarugi, P
AU  - Chiarugi P
FAU - Cirri, P
AU  - Cirri P
FAU - Taddei, L
AU  - Taddei L
FAU - Stefani, M
AU  - Stefani M
FAU - Raugei, G
AU  - Raugei G
FAU - Nordlund, P
AU  - Nordlund P
FAU - Ramponi, G
AU  - Ramponi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (Grb2 protein, mouse)
RN  - 0 (Isoenzymes)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
RN  - EC 2.7.10.2 (Oncogene Protein pp60(v-src))
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.1.3.48 (Acp1 protein, mouse)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - 3T3 Cells/metabolism
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - GRB2 Adaptor Protein
MH  - Glutathione Transferase/genetics/metabolism
MH  - Isoenzymes/chemistry/genetics/*metabolism
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Weight
MH  - Mutation
MH  - Oncogene Protein pp60(v-src)/immunology/metabolism
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Conformation
MH  - Protein Tyrosine Phosphatases/chemistry/genetics/*metabolism
MH  - Proteins/metabolism
MH  - *Proto-Oncogene Proteins
MH  - Receptors, Platelet-Derived Growth Factor/metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Tyrosine/*metabolism
MH  - src-Family Kinases/*metabolism
EDAT- 1999/08/19 00:00
MHDA- 1999/08/19 00:01
CRDT- 1999/08/19 00:00
PHST- 1999/08/19 00:00 [pubmed]
PHST- 1999/08/19 00:01 [medline]
PHST- 1999/08/19 00:00 [entrez]
AID - S0014-5793(99)00828-5 [pii]
AID - 10.1016/s0014-5793(99)00828-5 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jul 30;456(1):73-8. doi: 10.1016/s0014-5793(99)00828-5.