PMID- 10451922
OWN - NLM
STAT- MEDLINE
DCOM- 20000320
LR  - 20191024
IS  - 0965-1748 (Print)
IS  - 0965-1748 (Linking)
VI  - 29
IP  - 8
DP  - 1999 Aug
TI  - A 45-kDa cAMP-dependent phosphoprotein which is related to the product of Mst57Dc 
      in Drosophila melanogaster.
PG  - 701-10
AB  - In Drosophila, the catalytic subunit of cAMP-dependent protein kinase (PKA) is 
      preferentially expressed in the brain and the male reproductive organs. Although 
      the cAMP response element binding protein (CREB) is a major target of PKA in the 
      brain, the target of PKA in the male reproductive organs has been unknown. In the 
      present study, three cAMP-dependent phosphoproteins (referred to as pp45, pp20, 
      and pp10) were detected in the lumen fluid of male accessory glands. They were 
      tissue-specific secretory proteins that accumulated only after eclosion, and were 
      transferred to females during mating as other secretory proteins of the accessory 
      glands. Among them, the 45-kDa phosphoprotein was partially purified and 
      characterized. The purified protein was phosphorylated in vitro by the catalytic 
      subunit of PKA. The partial amino acid sequence of this 45-kDa phosphoprotein was 
      identical to the predicted amino acid sequence of the Mst57Dc cDNA, which is a 
      male accessory gland transcript.
FAU - Cho, K S
AU  - Cho KS
AD  - Department of Molecular Biology, Seoul National University, South Korea.
FAU - Lim, J H
AU  - Lim JH
FAU - Won, D H
AU  - Won DH
FAU - Gye, M C
AU  - Gye MC
FAU - Chung, K W
AU  - Chung KW
FAU - Lee, C C
AU  - Lee CC
LA  - eng
SI  - GENBANK/AF142325
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (DNA, Complementary)
RN  - 0 (Insect Proteins)
RN  - 0 (Phosphoproteins)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cyclic AMP
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - DNA, Complementary
MH  - *Drosophila melanogaster/genetics
MH  - Female
MH  - Insect Proteins/*genetics/metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Phosphoproteins/*genetics/metabolism
MH  - Phosphorylation
MH  - Sexual Behavior, Animal
EDAT- 1999/08/19 10:00
MHDA- 2000/03/25 09:00
CRDT- 1999/08/19 10:00
PHST- 1999/08/19 10:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 1999/08/19 10:00 [entrez]
AID - S0965174899000466 [pii]
AID - 10.1016/s0965-1748(99)00046-6 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 1999 Aug;29(8):701-10. doi: 
      10.1016/s0965-1748(99)00046-6.