PMID- 10449775
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 17
DP  - 1999 Aug 17
TI  - Targeted disruption of Traf5 gene causes defects in CD40- and CD27-mediated
      lymphocyte activation.
PG  - 9803-8
AB  - TRAF5 [tumor necrosis factor (TNF) receptor-associated factor 5] is implicated in
      NF-kappaB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase
      activation by members of the TNF receptor superfamily, including CD27, CD30,
      CD40, and lymphotoxin-beta receptor. To investigate the functional role of TRAF5 
      in vivo, we generated TRAF5-deficient mice by gene targeting. Activation of
      either NF-kappaB or c-Jun NH(2)-terminal kinase/stress-activated protein kinase
      by tumor necrosis factor, CD27, and CD40 was not abrogated in traf5(-/-) mice.
      However, traf5(-/-) B cells showed defects in proliferation and up-regulation of 
      various surface molecules, including CD23, CD54, CD80, CD86, and Fas in response 
      to CD40 stimulation. Moreover, in vitro Ig production of traf5(-/-) B cells
      stimulated with anti-CD40 plus IL-4 was reduced substantially. CD27-mediated
      costimulatory signal also was impaired in traf5(-/-) T cells. Collectively, these
      results demonstrate that TRAF5 is involved in CD40- and CD27-mediated signaling.
FAU - Nakano, H
AU  - Nakano H
AD  - Department of Immunology, Juntendo University, School of Medicine, 2-1-1 Hongo,
      Bunkyo-ku, Tokyo 113-8421, Japan. hnakano@med.juntendo.ac.jp
FAU - Sakon, S
AU  - Sakon S
FAU - Koseki, H
AU  - Koseki H
FAU - Takemori, T
AU  - Takemori T
FAU - Tada, K
AU  - Tada K
FAU - Matsumoto, M
AU  - Matsumoto M
FAU - Munechika, E
AU  - Munechika E
FAU - Sakai, T
AU  - Sakai T
FAU - Shirasawa, T
AU  - Shirasawa T
FAU - Akiba, H
AU  - Akiba H
FAU - Kobata, T
AU  - Kobata T
FAU - Santee, S M
AU  - Santee SM
FAU - Ware, C F
AU  - Ware CF
FAU - Rennert, P D
AU  - Rennert PD
FAU - Taniguchi, M
AU  - Taniguchi M
FAU - Yagita, H
AU  - Yagita H
FAU - Okumura, K
AU  - Okumura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CD40 Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Proteins)
RN  - 0 (TNF Receptor-Associated Factor 5)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 7)
RN  - 147205-72-9 (CD40 Ligand)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - CD40 Antigens/*immunology
MH  - CD40 Ligand
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Enzyme Activation
MH  - JNK Mitogen-Activated Protein Kinases
MH  - *Lymphocyte Activation
MH  - Membrane Glycoproteins/immunology
MH  - Mice
MH  - Mice, Knockout
MH  - *Mitogen-Activated Protein Kinases
MH  - NF-kappa B/metabolism
MH  - Proteins/*genetics
MH  - TNF Receptor-Associated Factor 5
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 7/*immunology
PMC - PMC22291
EDAT- 1999/08/18 00:00
MHDA- 1999/08/18 00:01
CRDT- 1999/08/18 00:00
PHST- 1999/08/18 00:00 [pubmed]
PHST- 1999/08/18 00:01 [medline]
PHST- 1999/08/18 00:00 [entrez]
AID - 10.1073/pnas.96.17.9803 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9803-8. doi:
      10.1073/pnas.96.17.9803.