PMID- 10449421
OWN - NLM
STAT- MEDLINE
DCOM- 19990927
LR  - 20161019
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 16
DP  - 1999 Aug 16
TI  - Purification and characterization of human RNPS1: a general activator of pre-mRNA
      splicing.
PG  - 4560-70
AB  - Biochemical purification of a pre-mRNA splicing activity from HeLa cells that
      stimulates distal alternative 3' splice sites in a concentration-dependent manner
      resulted in the identification of RNPS1, a novel general activator of pre-mRNA
      splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of
      unknown function, were previously identified in mouse and human. RNPS1 is
      conserved in metazoans and has an RNA-recognition motif preceded by an extensive 
      serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally
      synergizes with SR proteins and strongly activates splicing of both
      constitutively and alternatively spliced pre-mRNAs. We conclude that RNPS1 is not
      only a potential regulator of alternative splicing but may also play a more
      fundamental role as a general activator of pre-mRNA splicing.
FAU - Mayeda, A
AU  - Mayeda A
AD  - Department of Biochemistry and Molecular Biology, University of Miami School of
      Medicine, Miami, FL 33136, USA. amayeda@molbio.med.miami.edu
FAU - Badolato, J
AU  - Badolato J
FAU - Kobayashi, R
AU  - Kobayashi R
FAU - Zhang, M Q
AU  - Zhang MQ
FAU - Gardiner, E M
AU  - Gardiner EM
FAU - Krainer, A R
AU  - Krainer AR
LA  - eng
GR  - CA13106/CA/NCI NIH HHS/United States
GR  - HG01696/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (RNPS1 protein, human)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Rnps1 protein, mouse)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - DNA-Binding Proteins/isolation & purification/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Phosphoproteins/metabolism
MH  - Protein Processing, Post-Translational
MH  - RNA Precursors
MH  - *RNA Splicing
MH  - RNA-Binding Proteins/isolation & purification/*metabolism
MH  - Recombinant Fusion Proteins/isolation & purification/metabolism
MH  - *Ribonucleoproteins
MH  - Serine-Arginine Splicing Factors
MH  - Spodoptera
MH  - Subcellular Fractions
PMC - PMC1171530
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1093/emboj/18.16.4560 [doi]
PST - ppublish
SO  - EMBO J. 1999 Aug 16;18(16):4560-70. doi: 10.1093/emboj/18.16.4560.