PMID- 10449420
OWN - NLM
STAT- MEDLINE
DCOM- 19990927
LR  - 20161124
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 16
DP  - 1999 Aug 16
TI  - Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase 
      regulates spliceosome assembly.
PG  - 4549-59
AB  - Splicing factor 1 (SF1) functions at early stages of pre-mRNA splicing and
      contributes to splice site recognition by interacting with the essential splicing
      factor U2AF65 and binding to the intron branch site. We have identified an 80 kDa
      substrate of cGMP-dependent protein kinase-I (PKG-I) isolated from rat brain,
      which is identical to SF1. PKG phosphorylates SF1 at Ser20, which inhibits the
      SF1-U2AF65 interaction leading to a block of pre-spliceosome assembly. Mutation
      of Ser20 to Ala or Thr also inhibits the interaction with U2AF65, indicating that
      Ser20 is essential for binding. SF1 is phosphorylated in vitro by PKG, but not by
      cAMP-dependent protein kinase A (PKA). Phosphorylation of SF1 also occurs in
      cultured neuronal cells and is increased on Ser20 in response to a cGMP analogue.
      These results suggest a new role for PKG in mammalian pre-mRNA splicing by
      regulating in a phosphorylation-dependent manner the association of SF1 with
      U2AF65 and spliceosome assembly.
FAU - Wang, X
AU  - Wang X
AD  - Cell Signalling Unit, Children's Medical Research Institute, Locked Bag 23,
      Wentworthville 2145, NSW.
FAU - Bruderer, S
AU  - Bruderer S
FAU - Rafi, Z
AU  - Rafi Z
FAU - Xue, J
AU  - Xue J
FAU - Milburn, P J
AU  - Milburn PJ
FAU - Kramer, A
AU  - Kramer A
FAU - Robinson, P J
AU  - Robinson PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA Splicing Factors)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SF1 protein, human)
RN  - 0 (Sf1 protein, rat)
RN  - 0 (Splicing Factor U2AF)
RN  - 0 (Transcription Factors)
RN  - 0 (U2AF2 protein, human)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Brain/metabolism
MH  - Cattle
MH  - Cyclic GMP-Dependent Protein Kinases/*metabolism
MH  - *DNA-Binding Proteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Nuclear Proteins
MH  - Phosphorylation
MH  - Protein Kinases/metabolism
MH  - *RNA Splicing
MH  - RNA Splicing Factors
MH  - RNA-Binding Proteins/*metabolism
MH  - Rats
MH  - Ribonucleoproteins/*metabolism
MH  - Serine/*metabolism
MH  - Spliceosomes/*metabolism
MH  - Splicing Factor U2AF
MH  - Substrate Specificity
MH  - *Transcription Factors
PMC - PMC1171529
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1093/emboj/18.16.4549 [doi]
PST - ppublish
SO  - EMBO J. 1999 Aug 16;18(16):4549-59. doi: 10.1093/emboj/18.16.4549.