PMID- 10449094
OWN - NLM
STAT- MEDLINE
DCOM- 19990823
LR  - 20190826
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 36
IP  - 7
DP  - 1999 May
TI  - Biosynthesis of granulysin, a novel cytolytic molecule.
PG  - 413-22
AB  - Granulysin is a newly described lytic molecule expressed by CTL and NK cells.
      Three mRNA (519, 520, and 522) and two protein products of 15 and 9 kDa are
      encoded by the granulysin gene. Stable transfectants overexpressing the
      predominate 520 mRNA were generated to determine the protein products originating
      from the translation of this message. A transfectant of the NK cell tumor YT
      overexpressed both 15 and 9 kDa proteins while a transfectant of the T cell tumor
      HuT78 produced mainly 15 kDa granulysin. Thus the 520 mRNA is sufficient for
      production of both 15 and 9 kDa granulysin. 9 kDa granulysin accumulated via
      post-translational processing of 15 kDa protein and was present intracellularly
      but not in the cell culture supernatant, indicating specific retention of the 9
      kDa protein. An inhibitor of granule acidification, concanamycin A, blocked the
      processing of 15 kDa granulysin to the 9 kDa form. A deduced structural
      difference between the two forms of the protein and a decrease in lytic activity 
      of 9 kDa granulysin at granule pH suggest two mechanisms by which a granulysin
      expressing cell is protected from autolysis during the biosynthesis of this
      potentially harmful molecule.
FAU - Hanson, D A
AU  - Hanson DA
AD  - Department of Pediatrics, Stanford University School of Medicine, CA 94305-5208, 
      USA.
FAU - Kaspar, A A
AU  - Kaspar AA
FAU - Poulain, F R
AU  - Poulain FR
FAU - Krensky, A M
AU  - Krensky AM
LA  - eng
GR  - AI43348/AI/NIAID NIH HHS/United States
GR  - R01 DK35008/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (GNLY protein, human)
RN  - 0 (Macrolides)
RN  - 80890-47-7 (concanamycin A)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Antigens, Differentiation,
      T-Lymphocyte/*biosynthesis/chemistry/immunology/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Killer Cells, Natural/*chemistry/immunology
MH  - Kinetics
MH  - *Macrolides
MH  - Precipitin Tests
MH  - T-Lymphocytes, Cytotoxic/*chemistry/immunology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - S0161589099000632 [pii]
AID - 10.1016/s0161-5890(99)00063-2 [doi]
PST - ppublish
SO  - Mol Immunol. 1999 May;36(7):413-22. doi: 10.1016/s0161-5890(99)00063-2.