PMID- 10448098
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 1
DP  - 1999 Aug 19
TI  - Molecular cloning and functional characterization of a human homologue of
      centaurin-alpha.
PG  - 237-44
AB  - We report here the molecular cloning, expression, and characterisation of a human
      homologue of rat centaurin-alpha, which we have termed centaurin-alpha(1). The
      cDNA contains a single open reading frame, which encodes a 373-amino-acid protein
      with a calculated molecular weight of 43,429 Daltons. Centaurin-alpha(1) shows
      high identity at the amino acid level with the other centaurin-alpha homologues, 
      p42(IP4) and PIP(3)BP. Northern analysis revealed that centaurin-alpha(1)
      expresses as a single 2.5-kb transcript, mainly in the brain. Recombinant
      centaurin-alpha(1) binds the inositol head group of PtdIns(3,4,5)P(3) and
      Ins(1,3,4, 5)P(4), with high affinity (K(d) 139.7 +/- 10.5 nM) and inositol
      phosphate specificity, consistent with it functioning as a putative
      PtdIns(3,4,5)P(3) receptor. In keeping with this conclusion, we have shown that
      GFP-tagged centaurin-alpha(1) recruits to the plasma membrane in a PI
      3-kinase-dependent manner and the recruitment is inhibited by the PI 3-kinase
      inhibitor wortmannin. These results suggest that centaurin-alpha(1) can function 
      as an in vivo PtdIns(3, 4,5)P(3) receptor.
CI  - Copyright 1999 Academic Press.
FAU - Venkateswarlu, K
AU  - Venkateswarlu K
AD  - School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, United
      Kingdom.
FAU - Cullen, P J
AU  - Cullen PJ
LA  - eng
SI  - GENBANK/AJ006422
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (ADAP1 protein, human)
RN  - 0 (Adap1 protein, rat)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Androstadienes)
RN  - 0 (Carrier Proteins)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Inositol Phosphates)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphatidylinositol Phosphates)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (phosphatidylinositol 3,4,5-triphosphate)
RN  - 102850-29-3 (inositol-1,3,4,5-tetrakisphosphate)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Brain/metabolism
MH  - CHO Cells
MH  - Carrier Proteins/chemistry/*genetics/metabolism
MH  - Cell Membrane/drug effects/metabolism
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - GTPase-Activating Proteins
MH  - Humans
MH  - Inositol Phosphates/metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*genetics/metabolism
MH  - Organ Specificity
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphatidylinositol Phosphates/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - RNA, Messenger/analysis/genetics
MH  - Recombinant Fusion Proteins/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Wortmannin
MH  - Zinc Fingers
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1006/bbrc.1999.1065 [doi]
AID - S0006-291X(99)91065-3 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 19;262(1):237-44. doi:
      10.1006/bbrc.1999.1065.