PMID- 10448096
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 1
DP  - 1999 Aug 19
TI  - Molecular cloning and genomic organization of mouse homologue of Drosophila germ 
      cell-less and its expression in germ lineage cells.
PG  - 223-30
AB  - Primordial germ cells (PGCs) are founder cells of all gametes. A number of genes 
      which control PGCs development have been identified in invertebrates, whereas
      such genes are by and large unelucidated in mammals. Here we describe cloning,
      genomic structure and expression of mouse homologue of germ cell-less (gcl) gene 
      which is required for PGCs formation in Drosophila. The mouse gcl shows 34%
      identity compared with Drosophila gcl protein and contains BTB/POZ domain. The
      gcl gene consists of 14 exons and spans more than 50 kb. The CpG islands are
      found around exon 1 of the gene. Putative promoter region contains potential
      binding sites for various transcription factors. Northern blot analysis showed
      that its mRNA is highly expressed in adult testis with lower expression in ovary,
      ES (embryonic stem) cells, and various other organs. In situ hybridization
      analysis revealed strong expression of the gcl gene in the pachytene stage
      spermatocytes. The expression was also observed in post-migratory PGCs, but was
      not apparent in migratory and pre-migratory PGCs. Further studies including gene 
      disruption analysis would provide an important insight into mammalian germ
      lineage development.
CI  - Copyright 1999 Academic Press.
FAU - Kimura, T
AU  - Kimura T
AD  - Department of Molecular Cell Biology, Department of Science for Laboratory Animal
      Experimentation, Research Institute for Microbial Diseases, Osaka University,
      3-1, Yamada-oka, Suita-shi, Osaka, 565-0871, Japan. tkimura@biken.osaka-u.ac.jp
FAU - Yomogida, K
AU  - Yomogida K
FAU - Iwai, N
AU  - Iwai N
FAU - Kato, Y
AU  - Kato Y
FAU - Nakano, T
AU  - Nakano T
LA  - eng
SI  - GENBANK/AF163665
SI  - GENBANK/AF163666
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Drosophila Proteins)
RN  - 0 (Gcl protein, mouse)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (gcl protein, Drosophila)
SB  - IM
MH  - Aging
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Lineage/*genetics
MH  - Cell Movement
MH  - Cloning, Molecular
MH  - CpG Islands/genetics
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/genetics
MH  - Exons/genetics
MH  - Female
MH  - *Gene Expression
MH  - Germ Cells/cytology/*metabolism
MH  - Gonads/embryology/growth & development/metabolism
MH  - Intercellular Signaling Peptides and Proteins
MH  - Introns/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/*genetics/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Response Elements/genetics
MH  - Sequence Homology, Amino Acid
MH  - Stem Cells/cytology/*metabolism
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1006/bbrc.1999.1160 [doi]
AID - S0006-291X(99)91160-9 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 19;262(1):223-30. doi:
      10.1006/bbrc.1999.1160.