PMID- 10448092
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20091119
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 1
DP  - 1999 Aug 19
TI  - The DNA-binding and transcriptional activities of MAZ, a myc-associated zinc
      finger protein, are regulated by casein kinase II.
PG  - 198-205
AB  - Myc-associated zinc finger protein (MAZ) is a transcription factor that contains 
      proline-rich, alanine repeats and six C(2)H(2)-type zinc finger motifs, as well
      as five putative sites of phosphorylation by casein kinase II (CKII).
      Site-specific mutagenesis of MAZ revealed that the serine residue at position 480
      was the major site of phosphorylation by CKII both in vitro and in vivo.
      Phosphorylation of MAZ by CKII at this serine residue was required for maximum
      binding of MAZ to the pyrimidine-rich DNA of the nuclease-hypersensitive element 
      (NHE) in the 5'-end promoter region of the c-myc gene. Mutation of serine at
      position 480 to alanine eliminated the DNA-binding activity of MAZ to this
      element. Moreover, the mutated MAZ was unable to enhance the expression of
      luciferase encoded by a c-myc promoter/luciferase reporter gene in HeLa cells in 
      the presence of CKII. These results suggest that phosphorylation of the serine
      residue at position 480 of MAZ by CKII can control the function of MAZ by
      altering its DNA-binding activity.
CI  - Copyright 1999 Academic Press.
FAU - Tsutsui, H
AU  - Tsutsui H
AD  - Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical
      Research), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
FAU - Geltinger, C
AU  - Geltinger C
FAU - Murata, T
AU  - Murata T
FAU - Itakura, K
AU  - Itakura K
FAU - Wada, T
AU  - Wada T
FAU - Handa, H
AU  - Handa H
FAU - Yokoyama, K K
AU  - Yokoyama KK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (c-MYC-associated zinc finger protein)
RN  - 17885-08-4 (Phosphoserine)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Substitution
MH  - Casein Kinase II
MH  - Cell Nucleus/metabolism
MH  - DNA/genetics/metabolism
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - Genes, myc/genetics
MH  - HeLa Cells
MH  - Humans
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Response Elements/genetics
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - Transfection
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1006/bbrc.1999.1130 [doi]
AID - S0006-291X(99)91130-0 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 19;262(1):198-205. doi:
      10.1006/bbrc.1999.1130.