PMID- 10448091
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20181201
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 1
DP  - 1999 Aug 19
TI  - Sulfated tyrosines of thyroglobulin are involved in thyroid hormone synthesis.
PG  - 193-7
AB  - Thyroid hormone synthesis is under the control of thyrotropin (TSH), which also
      regulates the sulfation of tyrosines in thyroglobulin (Tg). We hypothesized that 
      sulfated tyrosine (Tyr[S]) might be involved in the hormonogenic process, since
      the consensus sequence required for tyrosine sulfation to occur was observed at
      the hormonogenic sites. Porcine thyrocytes, cultured with TSH but without iodide 
      in the presence of [(35)S]sulfate, secreted Tg which was subjected to in vitro
      hormonosynthesis with increasing concentrations of iodide. A 63% consumption of
      Tyr[S] (1 residue) was observed at 40 atoms of iodine incorporated into Tg,
      corresponding to a 40% hormonosynthesis efficiency. In addition, hyposulfated Tg 
      secreted by cells incubated with sodium chlorate was subjected to in vitro
      hormonosynthesis. With 0.5 Tyr[S] residue (31% of the initial content), the
      efficiency of the hormonosynthesis was 29%. In comparison, when hormonosynthesis 
      was performed by cells, with only 0.25 Tyr[S] residue (16% of the initial
      content), the hormonosynthesis efficiency fell to 18%. These results show that
      there exists a close correlation between the sulfated tyrosine content of Tg and 
      the production of thyroid hormones.
CI  - Copyright 1999 Academic Press.
FAU - Nlend, M C
AU  - Nlend MC
AD  - INSERM U38, Faculte de Medecine, Universite de la Mediterranee, 27 Bd J. Moulin, 
      Marseille, Cedex 05, 13385, France.
FAU - Cauvi, D
AU  - Cauvi D
FAU - Venot, N
AU  - Venot N
FAU - Chabaud, O
AU  - Chabaud O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chlorates)
RN  - 0 (Oligosaccharides)
RN  - 0 (Sulfates)
RN  - 0 (Thyroid Hormones)
RN  - 29166358BF (tyrosine O-sulfate)
RN  - 42HK56048U (Tyrosine)
RN  - 9002-71-5 (Thyrotropin)
RN  - 9010-34-8 (Thyroglobulin)
RN  - 9679TC07X4 (Iodine)
RN  - T95DR77GMR (sodium chlorate)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chlorates/pharmacology
MH  - Iodine/metabolism/pharmacology
MH  - Kinetics
MH  - Oligosaccharides/chemistry/metabolism
MH  - Sulfates/metabolism
MH  - Swine
MH  - Thyroglobulin/*chemistry/metabolism
MH  - Thyroid Gland/cytology/drug effects/metabolism
MH  - Thyroid Hormones/*biosynthesis
MH  - Thyrotropin/pharmacology
MH  - Tyrosine/*analogs & derivatives/metabolism
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1006/bbrc.1999.1173 [doi]
AID - S0006-291X(99)91173-7 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 19;262(1):193-7. doi:
      10.1006/bbrc.1999.1173.