PMID- 10448081
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 1
DP  - 1999 Aug 19
TI  - Computational EST database analysis identifies a novel member of the neuropoietic
      cytokine family.
PG  - 132-8
AB  - A novel member of the neuropoietic cytokine family has been cloned and the
      protein expressed and characterized. In an effort to identify novel secreted
      proteins, an algorithm incorporating neural network algorithms was applied to a
      large EST database. A full-length clone was identified that is 1710 bp in length 
      and has a single open reading frame of 225 amino acids. This new cytokine is most
      homologous to cardiotrophin-1, having a similarity and an identity of 46 and 29%,
      respectively, and therefore we have named it cardiotrophin-like cytokine (CLC).
      Northern hybridization analysis identified a 1.4-kb messenger RNA that is highly 
      expressed in spleen and peripheral leukocytes. Purified recombinant CLC induced
      the activation of NFkappaB and SRE reporter constructs in the TF-1, U937, and M1 
      cell lines. Furthermore, the signal transduction pathway for CLC was
      characterized in the neuroblastoma cell line SK-N-MC and found to involve
      tyrosine phosphorylation of gp130 and STAT-1.
CI  - Copyright 1999 Academic Press.
FAU - Shi, Y
AU  - Shi Y
AD  - Department of Molecular Biology, Department of Pharmacology, Department of
      Protein Expression, Human Genome Sciences, Inc., 9410 Key West Avenue, Rockville,
      Maryland 20850, USA.
FAU - Wang, W
AU  - Wang W
FAU - Yourey, P A
AU  - Yourey PA
FAU - Gohari, S
AU  - Gohari S
FAU - Zukauskas, D
AU  - Zukauskas D
FAU - Zhang, J
AU  - Zhang J
FAU - Ruben, S
AU  - Ruben S
FAU - Alderson, R F
AU  - Alderson RF
LA  - eng
SI  - GENBANK/AF172854
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (IL6ST protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (cardiotrophin-like cytokine)
RN  - 133483-10-0 (Cytokine Receptor gp130)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - AJ7U77BR8I (cardiotrophin 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, CD/metabolism
MH  - Cell Line
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Cloning, Molecular
MH  - Cytokine Receptor gp130
MH  - Cytokines/chemistry/*genetics/isolation & purification/pharmacology
MH  - DNA-Binding Proteins/metabolism
MH  - *Databases, Factual
MH  - *Expressed Sequence Tags
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - Humans
MH  - Membrane Glycoproteins/metabolism
MH  - Molecular Sequence Data
MH  - *Neural Networks, Computer
MH  - Phosphorylation/drug effects
MH  - Phosphotyrosine/metabolism
MH  - Phylogeny
MH  - RNA, Messenger/analysis/genetics
MH  - STAT1 Transcription Factor
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction/drug effects
MH  - Trans-Activators/metabolism
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1006/bbrc.1999.1181 [doi]
AID - S0006-291X(99)91181-6 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 19;262(1):132-8. doi:
      10.1006/bbrc.1999.1181.