PMID- 10448078
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 1
DP  - 1999 Aug 19
TI  - ZBP-99 defines a conserved family of transcription factors and regulates
      ornithine decarboxylase gene expression.
PG  - 113-20
AB  - Among transcription factors that regulate ornithine decarboxylase (ODC) gene
      expression are those that interact with GC-rich promoters, including Sp1 and
      ZBP-89. Sp1 functions as a transactivator and ZBP-89 as a transrepressor of both 
      the ODC and gastrin promoters. This study reports the cloning and
      characterization of a second member of the ZBP family that also binds GC boxes.
      ZBP-99 contains four Kruppel-type zinc fingers that collectively share 91% amino 
      acid sequence similarity and 79% sequence identity with those found in ZBP-89. In
      addition, there are highly conserved amino acid sequences in the carboxy-terminal
      segments of the two genes. In spite of their structural similarities, the two
      proteins are encoded at distinct loci, ZBP-89 on chromosome 3q21 and ZBP-99 on
      1q32.1. The predicted open reading frame of ZBP-99 cDNA encodes a 99-kDa protein.
      Electrophoretic mobility shift assays showed that ZBP-99 protein specifically
      binds to the GC-rich promoter elements of gastrin and ODC genes. Northern blot
      analysis showed that a major ZBP-99 transcript of 5.6 kb is expressed
      ubiquitously at low levels, with elevated expression levels in placenta and in
      adult kidney, liver, and lymphocytes. Cotransfection of AGS gastric
      adenocarcinoma and HT-29 colon adenocarcinoma cells with a ZBP-99 expression
      construct and with an ODC reporter construct show that ZBP-99 repressed basal
      expression in the two cell lines by 80 and 60%, respectively. Collectively, the
      data suggest that ZBP-99 binds GC-rich promoters and may complement the
      activities mediated by ZBP-89.
CI  - Copyright 1999 Academic Press.
FAU - Law, D J
AU  - Law DJ
AD  - Department of Internal Medicine, Department of Physiology, University of
      Michigan, Michigan, Ann Arbor 48109-0650, USA.
FAU - Du, M
AU  - Du M
FAU - Law, G L
AU  - Law GL
FAU - Merchant, J L
AU  - Merchant JL
LA  - eng
SI  - GENBANK/AF125158
GR  - DE-08229/DE/NIDCR NIH HHS/United States
GR  - DK-45729/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gastrins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (ZNF148 protein, human)
RN  - 0 (ZNF281 protein, human)
RN  - 9007-49-2 (DNA)
RN  - EC 4.1.1.17 (Ornithine Decarboxylase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Cloning, Molecular
MH  - Conserved Sequence/*genetics
MH  - DNA/genetics/metabolism
MH  - DNA-Binding Proteins/chemistry/genetics/metabolism
MH  - Gastrins/genetics
MH  - *Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Molecular Sequence Data
MH  - Ornithine Decarboxylase/*genetics
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Repressor Proteins/chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - *Trans-Activators
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - Tumor Cells, Cultured
MH  - Zinc Fingers
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1006/bbrc.1999.1180 [doi]
AID - S0006-291X(99)91180-4 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 19;262(1):113-20. doi:
      10.1006/bbrc.1999.1180.